ΜΕΛΕΤΗ ΤΗΣ ΕΚΦΡΑΣΗΣ ΤΟΥ ΑΝΘΡΩΠΙΝΟΥ ΕΜΒΡΥΟΝΙΚΟΥ Ε-ΣΦΑΙΡΙΝΙΚΟΥ ΓΟΝΙΔΙΟΥ

THE STUDY OF THE MOLECULAR MECHANISMS THAT TAKE PART IN THE REGULATION OF TRANSCRIPTION AND THE PROCESSING OF ITS PRODUCTS IS CENTRAL TO THE STUDY OF REGULATION OF GENE EXPRESSION. THE GLOBIN GENE SYSTEM IS ONE OF THE BEST STUDIED IN THIS DIRECTION. THE ANALYSIS OF TRANSCRIPTION AND RNA PROCESSING OF THE HUMAN EMBRYONIC E-GLOBIN GENE HAS BEEN STUDIED AND IS REPORTED IN THIS THESIS. WE ANALYZED TOTAL CELLULAR RNA FROM THE HUMAN HYBRID PUTKO CELL LINE AND FROM HELA CELLS TRANSIENTLY TRANSFECTED WITH THE E-GLOBIN GENE. FOR HELA CELL TRANSIENT EXPERIMENTS WE CONSTRUCTED A SERIES OF PLASMID VECTORS CONTAINING THE E-GLOBIN GENE AND, IN ALL POSSIBLE COMBINATIONS, THE SV40 ENHANCER SEQUENCES AND SV40 ORIGIN OFREPLICATION.

Grantor dc:publisher

National and Kapodistrian University of Athens

Year dc:date

1987

Authors dc:creator

• Kollias, Georgios
• Κόλλιας, Γεώργιος

• PUTKO ΚΥΤΤΑΡΑ
• Γονιδιακή έκφραση
• Γονιδιακή μεταφορά
• ΓΟΝΙΔΙΟ ΣΦΑΙΡΙΝΗΣ
• Ε-ΣΦΑΙΡΙΝΗ
• ΘΕΣΗ CAP
• Κ562 κύτταρα
• ΜΕΤΑΓΡΑΦΙΚΗ ΕΝΑΡΞΗ
• E-GLOBIN
• Gene expression
• Gene transfer
• GLOBIN GENE
• K562 CELLS
• PUTKO CELLS
• Φυσικές Επιστήμες
• Βιολογία
• Natural Sciences
• Biological Sciences

Language dc:language

gre

Identifier

10.12681/eadd/1749

OAI identifier oai:identifier

oai:10442/1749