Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 56 for “"SV40"”.
-
Studies of SV40-transformation and the Loss of Growth Factor Requirements
I found that SV40 transformation induced the loss of several specific growth factor requirements. In particular, SV40 transformed 3T3 fibroblasts had a significantly reduced growth requirement for insulin. A reduced insulin requirement was also observed in several other transformed cell lines. Dose …
-
ΜΕΛΕΤΗ ΤΗΣ ΕΠΙΔΡΑΣΗΣ ΤΟΥ ΘΕΡΜΙΚΟΥ ΣΟΚ ΣΕ ΜΟΛΥΣΜΕΝΑ ΚΑΙ ΜΕΤΑΣΧΗΜΑΤΙΣΜΕΝΑ ΑΠΟ ΤΟΝΙΟ SV40
… INDUCTION IN PERMISSIVE AND TRANSFORMED CELLS BY SV40 VIRUS. IT WAS SHOWN THAT PROTEINS OFTHE 70 KD FAMILY ARE INDUCED AFTER HEAT SHOCK (43 C) AND ARSENITE TREATMENT (20-25 MM) BUT THEY WERE ALSO INDUCED AFTER INFECTION OF THE CV1 CELLS WITH SV40 VIRUS. IT WAS ALSO SHOWN THAT THE ABOVE INDUCTION …
-
Regulation of melanogenesis in conditionally immortalised mouse melanocytes expressing a temperature-sensitive SV40 large T antigen
… carrying a temperature-sensitive mutant SV40 large T antigen. Six immortalised cell lines thus generated were analysed by northern and western blots and by enzymatic assays at the permissive temperature of the oncoprotein. Three epidermal and two dermal melanocyte clones remained …
-
Characterization of Tumorigenic Proteins
The 17K, 84K, and 94K SV40 T antigens from SV40 permissive and transformed cells were analyzed by tryptic peptide mapping on ion exchange columns. The tryptic peptides were mapped relative to the SV40 DNA and to their known functions. The aminotermini of the 17K, 84K and 94K T antigens are …
-
Purification and Characterization of Simian Virus 40 Large T Antigen
… of evidence indicate that large T antigen of SV40 is involved in the initiation of viral DNA replication during productive infection, the stimulation of host cell DNA synthesis following SV40 infection, the regulation of viral transcription, and the initiation and maintenance of …
-
ΜΕΛΕΤΗ ΤΗΣ ΕΚΦΡΑΣΗΣ ΤΟΥ ΑΝΘΡΩΠΙΝΟΥ ΕΜΒΡΥΟΝΙΚΟΥ Ε-ΣΦΑΙΡΙΝΙΚΟΥ ΓΟΝΙΔΙΟΥ
… GENE AND, IN ALL POSSIBLE COMBINATIONS, THE SV40 ENHANCER SEQUENCES AND SV40 ORIGIN OFREPLICATION.
-
The Role of IGF-1 and Notch Signaling in Thoracic Malignancies.
… or MM). The DNA oncogenic virus Simian Virus 40 (SV40) has been shown to cooperate with environmental oncogenic fibers in the onset of MM. Insulin like growth factor-1 (IGF-1) signaling plays a central role in all thoracic malignancies and in the process of SV40-mediated malignant transformation …
-
Einfluß des IL-6/sIL-6R Fusionsproteins Hyper Interleukin-6 auf die Leber nach hydrodynamischer Transfektion in vivo
… Höhe und Dauer) zwischen 1 ng/ml (SV40, hAAT) und 1000 ng/ml (CMV) für 1-2 Tage (CMV, SV40) oder 6-9 Tage (hAAT) in C57 Bl/6 Mäusen durch hydrodynamische Transfektion in vivo erreicht werden. Durch die Wahl des Promotors (CMV, SV40, hAAT) konnte die Expressionskinetik des Transgens …
-
A Role for Oligomerization in the Multifunctional Nature of the Simian Virus 40 Large T Antigen Protein (Tumor, Dna, Cell Transformation, Association)
Simian virus 40 (SV40) large T antigen protein plays a central role in the interaction of the virus with its host. It is required for both the growth of the virus in permissive hosts and in the transformation of non-permissive hosts to a tumorigenic phenotype. Biochemical studies reveal that large …
-
Microphysiometry Studies of Rapid Binding of Insulin-Like Growth Factor I by Parental and Transfected Mammary Epithelial Cell Lines
… This study examines the IGF-I response of SV40-IGF-I, an immortalized bovine mammary epithelial cell line which secretes IGF-I constitutively. The microphysiometer allows real-time sampling of cellular activity by measuring the excretion of protons from a sample of cells stimulated by IGF-I …
-
Zur Transkriptions- und Translationskontrolle des Gens für Transitionsprotein
… the translational repression of Tnp2 mRNA, Tnp2-SV40 transgenic ice line was generated where the 3"UTR of Tnp2 of gene was replaced by SV40 splicing and polyadenlylation sequences to abolish the translational delay for the Tnp2 RNA. An interesting aspect of spermatogenesis is the translational …
-
An in vitro model of mouse corneal endothelial development Mohammed Mubeen Goolam.
… E13.5 mouse embryos and immortalised with the SV40 large T-antigen. Two cell lines were thus derived and named E12.5POM and E13.5POM respectively.
-
The role of anchorage in cell cycle control
… potential. Here I have used cells expressing SV40 LT, with and without oncogenic Ras, to model the changes leading to anchorage independent proliferation. Importantly, cells expressing SV40 LT alone retain their anchorage dependence, despite Rb and p53 inactivation. However, the mechanism …
-
Altered DNA Repair, Antioxidant and Cellular Proliferation Status as Determinants of Susceptibility to Methylmercury Toxicity in Vitro
… using: (1) spontaneously- and Simian virus 40 (SV40) large T antigen-immortalized oxoguanine glycosylase 1-null (Ogg1-/-) murine embryonic fibroblasts (MEFs); and, (2) human Ogg1 (hOgg1)- or formamidopyrimidine glycosylase (Fpg)-expressing human embryonic kidney (HEK) cells; reciprocal in vitro …
-
Epigenetic Patterns: Process-Driven Or Sequence-Driven?
… the effect of novel sequence inserts into the SV40 genome related to the enhancer region, Sp1 binding site, and late promoter. Specific variants of SV40 were created to contain either one or two copies of the enhancer region (T-129 and T-143 respectively), two copies in succession of the Sp1 …
-
Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… repeat (pMMTV-IGF-I), early simian virus (pSV40-IGF-I), and herpes simplex thymidine kinase (pTK-IGF-I) promoters. Stably transfected clones were generated by cotransfection of clonal MAC-T cells with the IGF-I expression vectors and a plasmid conferring resistance to hygromycin-B (HYG-B), …
-
The susceptibility of primordial germ cells to malignant transformation and isolation and characterization of members of a new gene family differentially expressed in invasive and non-invasive immortalized male germ cells
… Mausmodell. In diesem Modell verwendeten wir das SV40 large Tumor Antigen (LTA),welcher mit dem Keimzellen alkalische Phosphatase (GCAP) Promotor verbunden ist, um direkt die Onkogenische Aktivität von SV40-LTA hinsichtlich PGCs spezifischally zu richten. Menschliche GCAP sich entwicklungsmässig …
-
DESIGN AND INVESTIGATION OF DUMBBELL-SHAPED DNA MINIMAL VECTORS FOR CRISPR/CAS9 EDITING
… helper functions enhancing DNA nuclear import (SV40 enhancer sequence) and Cas9 mRNA nuclear export (WPRE), improved the editing efficiency of both plasmid and dumbbell vectors. Further, we developed the first genetic 3-in-1 editing vectors comprising sgRNA and Cas9 plus a double- or …
Page 1 of 3