Καλλιέργεια λεμφοκυττάρων ευαισθητοποιημένων στον εχινόκοκκο ατόμων παρουσία προϊόντων υδατιδικής κύστεως: Βλαστική μεταμόρφωση λεμφοκυττάρων και δράση επί των σκωληκοκεφαλών

The purpose of this work was: (1) to investigate the immune memory of lymphocytes from subjects with active or cured echinococcosis, and (2) to determine whether or not sensitized lymphocytes can kill E. granulosus scolices in vitro. Monocytes, mainly lymphocytes, were isolated by the Ficoll-Hypaque system and cultivaled in RPMI-1640. The immune response of the lymphocytes was studied by adding in the culture medium hydatid fluid and measuring the [³H] thymidine incorporation into the DNA of the cells. The viability of scolices and lymphocytes was determined by the Trypan Blue exclusion technique. All experiments were carried out in triplicate. The findings of the study demonstrate: 1. Lymphocytes from patients with echinococcosis are stimulated when cultured in the presence of hydatid fluid. Maximum stimulation occurred on the sixth day of culture, at a hydatid fluid protein concentration of 15 μg per ml of culture medium. The stimulation Index (SI) of the lymphocytes obtained from 10 subjects with active or cured echinococcosis was 4.3 ± 1.6 (mean ± SD), with a range from 7.0 to 2.1. Lymphocytes from normal control subjects demonstrated a SI of 1.1 ± 0.3, with a range from 0.5 to 1.5. 2. The immune memory of the sensitized lymphocytes is maintained at least up to 20 years after removal of the hydatid cyst, even if the Casoni's skin test and serology have become negative. 3. The viability of E. granulosus scolices cultivated in the presence of sensitized lymphocytes was significantly lower than that of the same scolices cultivated in the presence of nonsensitized lymphocytes. On the 5th day of culture only 5% of the scolices cocultivated with sensitized lymphocytes were alive, whereas on the same day of culture 57%, of the same scolices cocultlvated under similar conditions with nonsensitized lymphocytes were alive. 4. Sensitized lymphocytes were killed by E. granulosus scolices to a much greater extend than nonsensitized lymphocytes. 5. The reduced viability of both sensitized lymphocytes and scolices, when cocultivated, was not the result of a nutrient depletion, but was caused by the lymphocyte immune response. 6. The findings of this study indicate the presence long-lasting cellular immunity in echinococcosis and suggest that the production of a vaccine against E. granulosus is feasible.

Grantor dc:publisher

University of Patras

Year dc:date

1990

Authors dc:creator

• Moustogianni-Kallimani, Anastasia
• Μουστόγιαννη-Καλλιμάνη, Αναστασία

• Βλαστική μεταμόρφωση
• Ευαισθητοποιημένα λεμφοκύτταρα
• Εχινοκοκκικό αντιγόνο
• Εχινόκοκκος granulosus
• Καλλιέργειες
• Κυτταρική ανοσία
• Λεμφοκυτταρική διέγερση
• Λεμφοκυτταρική μνήμη
• Πρωτοσκώληκες
• Υδατίδα κύστη
• Blast transformation
• Cellular immunity
• Culture
• Echinococcus granulosus
• Hydatid fluid
• Hydatid fluid's antigen
• Immune memory
• Lymphocytes stimulation
• Protoscolices
• Sensitized lymphocytes
• Ιατρική και Επιστήμες Υγείας
• Κλινική Ιατρική
• Medical and Health Sciences
• Clinical Medicine

Language dc:language

gre

Identifier

10.12681/eadd/1497

OAI identifier oai:identifier

oai:10442/1497