Ο ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΤΗΣ ΠΡΩΤΕΙΝΗΣ ΡΟΥΜΠΙΣΚΟ (RUBISCO) ΣΤΑ ΦΥΤΑ

THE PRESENT STUDY WAS CONDUCTED TO FIX A RELIABLE METHOD OF QUANTITY DETERMINATION OF RUBISCO PROTEIN IN TOBACCO CRUDE LEAF EXTRACT IN ORDER TO ESTIMATE THE VARIATION OF RUBISCO PROTEIN CONTENT IN 15 TOBACCO CULTIVARS AND THE OPTIMUM TYPE OF HARVEST FOR THE MAXIMIZATION OF ITS EXTRACTION. ROCKET IMMUNOELECTROPHORESIS TECHNIQUE SEEMED TO BE THE MOST ACCURATE AND APPROPRIATE METHOD BECAUSE IT MAKES POSSIBLE THE QUANTITATION OF THE TOTAL EXTRACTABLE RUBISCO AND NOT ONLY THE ENZYMATICALLY ACTIVE, IN MIXTURE WITHOUT A PREVIOUS PURIFICATION OF THE PROTEIN . HIGHLY PURIFIED RUBISCO IN CRYSTALLINE FORM WAS EXTRACTED FROM TOBACCO LEAVES AND WAS USED AS ANTIGEN FOR THE ANTIBODY PRODUCTION. THE TITRATION OF ROCKET IMMUNOELECTROPHORESIS FOLLOWED AND THE APPLICATION FOR THE DETERMINATION OF OPTIMUM TIME OF HARVEST AND THE ESTIMATION OF VARIATION OF 15 GREEK TOBACCO CULTIVARS FOR RUBISCO AND POLYPHENOL CONTENT AS WELL. OPTIMUM TIME OF HARVEST FOR THE EXTRACTION WAS FOUND TO BE THE AGE OF 2,5 MONTHS AFTER TRANSPLANT. AT THE IMMATURE STAGE THE PROTEIN LEVEL WAS CONSIDERABLY HIGHER THAN THAT OF THE MATURE OR OVERMATURE STAGES OF LEAVES. BESIDES, THE LEAVES OF THE TOP POSITIONS GIVE HIGHER RUBISCO CONCENTRATIONS RELATIVE TO THOSE OF LOWER POSITIONS. (SHORTENED)

Grantor dc:publisher

Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ)

Year dc:date

1990

Author dc:creator

• Χασιώτου-Κουιμτζή, Ευθυμία

• ANTISERUM
• POLYPHENOL DETERMINATION
• RUBISCO PURIFICATION
• TOBACCO RUBISCO
• ΑΝΟΣΟΓΛΟΒΟΥΛΙΝΕΣ
• ΚΑΘΑΡΙΣΜΟΣ ΡΟΥΜΠΙΣΚΟ
• ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΠΟΛΥΦΑΙΝΟΛΩΝ
• ΡΟΥΜΠΙΣΚΟ ΚΑΠΝΟΥ
• Γεωπονικές Επιστήμες και Κτηνιατρική
• Agricultural and Veterinary Sciences
• Γεωπονία, Δασολογία και Αλιεία
• Agriculture, Forestry and Fisheries

Language dc:language

gre

Identifier

10.12681/eadd/1461

OAI identifier oai:identifier

oai:10442/1461