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Showing 1 to 20 of 78 for “"RuBisCO"”.
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Mechanism of Light Regulation of Rubisco by Rubisco Activase via Thioredoxin -F in Arabidopsis Thaliana
Rubisco activase is a nuclear-encoded chloroplast protein that is required for the light activation of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) in vivo. In most plants examined to date, there are two soforms of Rubisco activase arising from alternative splicing that differ only at …
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Interactions of ATP, ADP and magnesium ion with rubisco activase and their effects on rubisco activation
Rubisco activase is a chloroplast protein that mediates a greatly enhanced activation of rubisco in the presence of ATP and Mg$\sp{2+}$. The fluorescent dye 1-anilinonaphthalene-8-sulfonate was used to study the binding of rubisco activase with ligands. The results indicated that rubisco activase …
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Regulation of Rubisco in Arabidopsis Thaliana Rca(-) Complemented With Arabidopsis Rubisco Activase Engineered at an ATP Binding Site
Q111E and Q111D showed rates of Rubisco activation greater than 42kD wild type and Q111S at ADP/ATP ratios known to exist in planta. Q111D showed a Rubisco activation rate at night time ADP/ATP greater than the 42kD wild type rate at zero ADP/ATP suggesting an altered response. Measurements of …
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Ο ΠΡΟΣΔΙΟΡΙΣΜΟΣ ΤΗΣ ΠΡΩΤΕΙΝΗΣ ΡΟΥΜΠΙΣΚΟ (RUBISCO) ΣΤΑ ΦΥΤΑ
… A RELIABLE METHOD OF QUANTITY DETERMINATION OF RUBISCO PROTEIN IN TOBACCO CRUDE LEAF EXTRACT IN ORDER TO ESTIMATE THE VARIATION OF RUBISCO PROTEIN CONTENT IN 15 TOBACCO CULTIVARS AND THE OPTIMUM TYPE OF HARVEST FOR THE MAXIMIZATION OF ITS EXTRACTION. ROCKET IMMUNOELECTROPHORESIS TECHNIQUE SEEMED …
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Functional discovery and promiscuity in the RuBisCO superfamily
… 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) catalyzes the carboxylation of D-ribulose 1,5-bisphosphate to yield two molecules of 3-phosphoglycerate. Currently, there are four forms of RuBisCO, three of which catalyze the canonical carboxylation reaction. Form IV RuBisCOs (also known …
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Molecular characterization of phosphoribulokinase and rubisco activase from Chlamydomonas reinhardtii
… indicated the presence of a single Chlamydomonas rubisco activase polypeptide which possessed 60 to 65% amino acid sequence identity with the higher plant polypeptides. Chlamydomonas rubisco was less effectively activated by spinach rubisco activase than was spinach rubisco. Genomic DNA blot …
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Creation and analysis of chimeras of spinach and tobacco Rubisco activase
Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco: EC 4.1.1.39) catalyzes the initial step in photosynthesis, by combining CO$\sb2$ with ribulose bisphosphate to form two molecules of PGA. Rubisco activase is a protein which regulates the activity of Rubisco in vivo and hydrolyzes ATP. …
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Assignment of Enzyme Function Through Characterization of the RuBisCO and Enolase Superfamilies
The second superfamily explored in this work is that of the enolase superfamily. Genomic context and primary amino acid sequence make it clear that although the enzymes in this superfamily are structurally and mechanistically related, the chemistry and substrates are varied. Two highly divergent …
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Site-directed mutagenesis of the ATP-binding site in spinach rubisco activase
Spinach rubisco activase has two isoforms derived from alternate splicing with the larger polypeptide containing an additional 4 kD C-terminal domain. Both isoforms, with molecular masses of 41 kD and 45 kD, were synthesized and purified from the transformed E. coli containing the cDNAs. A …
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Role of the small subunit of Rubisco in the green algal phylogeny and Carbon Concentrating Mechanism expression
… is also 8,000 times slower. In addition, Rubisco, which catalyses the first major step of carbon fixation, converting atmospheric CO2 into precursors of energy-rich molecules, exhibits slow catalytic rates, low affinity for CO2 and competition from O2 for the active sites. Therefore, most …
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Fluorination of Rubisco-Mimetic CO2 Capture Systems. Theoretical and Experimental Studies of Ammonium Ion Acidity Depression and Carbamylation
<p>"RuBisCO-inspired CO<sub>2</sub> capture and release (CCR) systems featuring amines have been developed for the purpose of reversable CO<sub>2</sub> capture from air. The enzyme active site consists of the tetrapeptide sequence Lys-Asp-Asp-Glu. The Lys sidechain amine undergoes carbamylation and …
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Identifying the Rubisco Recognition Domain and Critical Arginines in Activase and Engineering Activase With Improved Thermostability for Arabidopsis
Heat sensitivity of Rubisco activase(s) confers a major limitation on leaf photosynthesis at moderately high temperature in Arabidopsis. To overcome this limitation, a thermostable activase for Arabidopsis was generated by engineering the more thermostable tobacco activase with an Arabidopsis …
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Rubisco Activase -- a Typical AAA+ Protein With Unique Features, And, PIFI -- a Novel Chloroplast Protein Functioning in Chlororespiration
A novel Arabidopsis chloroplast protein, named PIFI, with a similar C-extension as activase was identified as functioning in chlororespiration and possibly cyclic electron transport by reverse genetics. In a pifi mutant, which possesses an intact NDH complex, NDH-mediated dark reduction of …
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Molecular Physiology of the Chlamydomonas Pyrenoid
… *Chlamydomonas reinhardtii* relies on confining Rubisco in a proteinaceous microcompartment, the chloroplastic pyrenoid. Despite a long history of research, this enigmatic structure lacks a full definition both in terms of physiological function as well as molecular composition. The work …
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Isolation, characterization and expression ofcDNA and genomic sequences encoding ribulosebisphosphate carboxylase/oxygenase activase from barley
… carboxylase/oxygenase (EC 4.1.1.39, rubisco) catalyzes either the carboxylation or oxygenation of ribulose-1,5-bisphosphate (RuBP) to initiate either the Calvin cycle or the photorespiratory pathway. Rubisco needs to be activated in order to catalyze these reactions. Rubisco …
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Transgenic approaches to improve photosynthesis and nitrogen use in wheat
… leaf rate and total biomass production. The Rubisco protein is a major N investment in crops, but it has also been exhibited that under some environmental conditions, there may be an excess of Rubisco greater than that needed to maintain photosynthesis. This raises the question of whether a …
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Structure and expression of Spinacea oleracea L. and Arabidopsis thaliana complementary-DNAs encoding ribulosebisphosphate carboxylase/oxygenase activase
Rubisco activase proteins consist of two immunologically related polypeptides in the range of 40- to 47-kDa in most plant species examined. Rubisco activase precursor polypeptides are synthesized on cytoplasmic ribosomes and post-translationally imported into the chloroplast with cleavage of a …
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Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid
… Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Many eukaryotic algae, and a single group of land plants, the hornworts, have an inducible carbon concentrating mechanism (CCM), to overcome these limitations. The efficiency of the CCM is improved when RuBisCO is localised to a …
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Structure-function relationships for the small subunit (S) of ribulose-1,5-bisphosphate carboxylase/oxygenase: Foreign S expression and characterization of engineered protein
… Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) (EC4.1.1.39) might influence Rubisco function. Toward this analysis a pea RbcS 3A cDNA expression cassette was introduced into Arabidopsis thaliana Landsberg by Agrobacterium tumefaciens mediated transformation. Analysis of RNA blots and …
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Improving the thermal tolerance of photosynthesis in wheat
… due to a decrease in the activation state of Rubisco. Rubisco activase (Rca) is required to restore and maintain the catalytic activity of Rubisco. Rca has a relatively low temperature optimum; improving its thermal tolerance would maintain Rubisco activity and enhance photosynthesis at higher …
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