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Showing 1 to 20 of 113 for “"ANTISERUM"”.
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Identification of an aqueous glue protein, SCP-2, and the development of a polyclonal antiserum against the bHLH transcription factor SGSF in Latrodectus Hesperus
… were injected into rabbits and the polyclonal antiserum was collected and tested by western blot analysis to determine the specificity of the immunological reagent. Western blot analyses revealed the anti-SGSF antiserum was capable of recognizing bacterially expressed SGSF in an efficient …
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Detection of filamentous bacteria in activated sludge
… success was only achieved with the latter. The antiserum reacted specifically with S. natans. Subsequently, existing microscopic techniques for the identification of filamentous bacteria occurring in activated sludge were compared with the use of the S. natans fluorescent antiserum. The …
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A study of treponemal antigens
… also reacted with rabbit fibronectin antiserum and rabbit albumin antiserum in immunodiffusion and ELISA assays. Three proteins with molecular weight of 60K, 47K, and 21K, were eluted from a agarose-gelatin-fibronectin column and reacted in Wester blots with T. denticola antiserum.
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Study of macroglobulinaemia in Trypanosoma equiperdum infections of the rabbit
… of rabbit IgM and IgG: i. goat anti rabbit IgM antiserum; ii. goat anti rabbit IgG antiserum; iii. goat antiserum to rabbit serum proteins. 2. The serum levels of 195 IgM, measured by gel diffusion and rocket immunoelectrophoresis were shown to increase approximately ten times at the peak of the …
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Isolation and detection of bean yellow mosaic, clover yellow vein and peanut stunt viruses from Trifolium L. species
… Washington Counties were positive against CYVV antiserum and four white clover samples from Augusta County were positive against PSV antiserum. Single red clover samples from Frederick and Montgomery Counties were positive against BYMV antiserum. There were notable differences in host range with …
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Immunological investigations into synaptic plasticity
… injection of an anti-postsynaptic density antiserum 2) mapping time- and locus-specific changes in the chick brain using antisera to the cytoskeletal proteins (Xtubulin and microtubule-associated protein 2 following passive avoidance training and 3) using antibodies to synaptically-enriched …
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The relationship between two forms of glycogen phosphorylase in Dictyostelium discoideum
… enzyme show low cross reactivity. The anti-’a' antiserum reacts with a 104 kd protein that is associated with phosphorylase ’a' activity; the anti-’b' antiserum reacts with a 92 kd protein that is associated with phosphorylase ’b' activity and cross reacts weakly with the 104 kd protein. Cyclic …
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Immuno-gluorescence study of five zygomycetous fungi with two chitin-binding probes
A polyclonal antiserum was prepared against a purified microsomal chitinase isolated from the fungus Choanephora cucurbitarum. Indirect immunofluorescence was used to localize chitinase at various developmental stages of five zygomycetous fungi and during abiotrophic mycoparasite interaction with a …
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Charakterisierung der biologischen Aktivität von Hühner Interleukin-6 und erste Untersuchungen zum Toll-like Rezeptor-System des Huhnes
… wurde ein neutralisierendes, polyklonales ChIL-6 Antiserum entwickelt. Durch wiederholte Vakzinierung von Kaninchen mit rChIL-6 (E.coli) war es möglich, ein ChIL-6 Antiserum zu gewinnen, welches auch die biologische Aktivität von natürlichem ChIL-6 neutralisiert. In vivo induzierte die intravenöse …
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Transcriptional and Translational Products of Avian Erythroblastosis and Avian Myeloblastosis Viruses
… proteins are closely related to each other. An antiserum specific to the unique sequences in AEV p75 was prepared by immunizing chickens that had regressed from erythroblastic leukemia induced by a temperature-sensitive mutant of AEV, AEVts34. This antiserum did not cross-react with p46/p48 …
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Anti-idiotype antibodies and phage displayed peptides as antigenic mimics of a Mycoplasma capricolum subsp. capripneumoniae epitope
… were not detectable using Mccp-specific goat antiserum suggesting that the serum did not contain paratopes that could accommodate the surrogate epitopes. In spite of this, the antiserum efficiently inhibited the binding of the Mab to immobilised mycoplasmal lysate in a standardised test for …
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Purification and characterization of Clostridium perfringens iota toxin
… iota or iota-like toxin. By using C. spiroforme antiserum and crossed immunoelectrophoresis (crossed IEP), we found two cross-reacting antigens in C. perfringens type E supernatants. C. perfringens types A, B, C, and D, which do not produce iota toxin, did not cross-react with C. spiroforme …
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Immunoassays with chemically modified bacteriophage
… the inactivation of native T4 phage by phage antiserum and anti phage IgG, the chemical modification of phage with DNP and the attachment of lysozyme to phage under a variety of conditions. The success of the covalent binding of protein to phage particles depends on finding conditions under …
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Antibody Dependent Enhancement of Visceral Leishmaniasis
… to verify cross reactivity of <em>L. major</em> antiserum to antigen derived from both <em>L. major</em> and <em>L. infantum</em>. Results indicate substantial cross reactivity to the two antigens. This was followed with phagocytosis assays to measure parasite uptake by macrophages <em>in …
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Immuno-histochemical method for demonstration of Lactic Dehydrogenase "M" subunits in Rat Skeletal Muscle, Kidney and Peripheral Nerve
… phase fixation of freeze-dried sections. An antiserum to monkey LDH-M^ was found to cross react with rat LDH-M subunits. The fibers of the gastrocnemius and soleus muscles which are stained using the immuno-peroxidase procedure do not stain in the presence of 4 M urea in the conventional …
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Isolation and analysis of a corprinus cinereus DNA fragment showing homology to a fimbrial cDNA from ustilago violacea
… fungal classes. In the present study, polyclonal antiserum raised against the fimbrial proteins of U. vio/acea were used in order to identify antigenically related proteins from Coprinus cinereus and Schizophy//um commune. Two polypeptides with molecular masses of 37 and 39 kDa from C. cinereus …
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Expression of Bacillus Anthracis Protective Antigen in Vaccine Strain Brucella Abortus Rb51
… to express in E. coli by immunoblotting using antiserum specific for PA. The immunoblot results revealed that E. coli produced a PA protein of the expected size. In addition, the culture medium was shown to contain the same PA protein using immunoblotting. These results show that E. coli is …
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Investigation of a Possible Multi-enzyme Complex Involved in Nicotine Biosynthesis in Roots of Tobacco (Nicotiana tabacum)
… culture extracts was used to generate immune antiserum in rabbits (McLauchlan et al., 1993). In an attempt to clone a cDNA encoding MPO, we used this antiserum to screen a tobacco cDNA expression library. Unexpectedly, two previously unreported genes with strong homology to members of a gene …
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Analysis of a trichomonas vaginalis cysteine protease
… were developed to produce CP1 to generate antiserum, and to have enough active protein for biochemical characterization. Secondly, endogenous and epitope tagged CP1 were localized in T. vaginalis vesicles. These vesicles were confirmed to have alkaline phosphatase activity which is a …
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