University of Ioannina
ΜΕΛΕΤΗ ΤΗΣ ΠΡΩΤΕΑΣΗΣ ΚΑΙ ΛΙΠΑΣΗΣ ΨΥΧΡΟΤΡΟΦΟΥ ΒΑΚΤΗΡΙΑΚΟΥ ΣΤΕΛΕΧΟΥΣ ΤΟΥ ΝΩΠΟΥ ΓΑΛΑΚΤΟΣ ΚΑΙ ΕΠΙΔΡΑΣΗ ΤΟΥΣ ΣΤΗΝ ΠΟΙΟΤΗΤΑ ΤΟΥ ΤΥΡΙΟΥ ΦΕΤΑ
Abstract
dc:descriptionIN THIS THESIS A PSEUDOMONAS FLUORESCENS STRAIN FROM RAW MILK WAS ISOLATED AND CHARACTERIZED. THE EFFECT OF CULTURE CONDITIONS ON THE PRODUCTION OF THE EXTRACELLULAR PROTEASE AND LIPASE FROM THE BACTERIUM WAS STUDIED. THE ENZYMES WERE PURIFIED AND THEIR PROPERTIES WERE STUDIED. THE ACTION OF PROTEASE ON THE SIMULTANEOUSLY PRODUCED LIPASE WAS EXAMINED. THE BEHAVIOR OF THE TWO ENZYME DURING THERMAL TREATMENT WAS STUDIED. THE EFFECT OF THE PSYCHROTROPHIC STRAIN (VIA ITS ENZYMES) ON THE QUALITY OF FETA CHEESE PRODUCED BASED ON CONSTITUENT ANALYSIS ANDCOMPARISON WITH CONTROL CHEESE WAS STUDIED. THE MOST IMPORTANT RESULTS ARE AS FOLLOWS: 1. THE BACTERIUM CHARACTERIZED TO BE PSEUDOMANS FLUORESCENS, 2. THE PRODUCTION AND ACTIVITY OF THE ENZYMES WERE SIGNIFICANT UNDER "INDUSTRIAL" CONDITIONS, 3. THE PROTEASE WAS CHARACTERIZED TO BE AS THIOL ENZYME AND CONTAIN CU AND CA IN ITS MOLECULE, 4. THE LIPASE SEEMED TO BE SERINE ENZYME WITH POSSIBLE PARTICIPATION OF CA2+ IN ITS CATALYTIC ACTIVITY, 5. THE PROTEASE REDUCED THE ACTIVITY OF THE LIPASE PROBABLY THROUGH HYDROLYSIS, 6 . FROM THE STUDY OF THE BEHAVIOUR OF THE TWO ENZYMES DURING THERMAL TREATMENT IN CONJUCTION WITH RESULTS OF OTHER WORKERS A MODEL ON THIS BEHAVIOR IS PROPOSED, 7. THE ACTION OF THE ENZYMES MAY LEAD IN SIGNIFICANT INCREASE/DECREASE OF FETA CONSTITUENT LEVELS AS WELL AS THE PRODUCTION OF NEW ONES, 8. THE POPULATION VALUE FOR PSYCHROTROPHS OF 5 X10 6 CFU/ML WHICH HAD BEEN PROPOSED FOR THE REJECTION OF MILK MAY NOT BE OF GENERAL APPLICATION.
Degree
thesis:*- Grantor dc:publisher
- University of Ioannina
- Year dc:date
- 1987
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Ρούσσης, Ιωάννης
Subjects
dc:subject × 12Rights
- Language dc:language
- gre
Identifiers
dc:identifier.*- Identifier
- 10.12681/eadd/0596
- OAI identifier oai:identifier
- oai:10442/0596