Expression and Characterization of Putative Plant Secondary Product Glucosyltransferase Clone 10 from <em>Citrus paradisi</em> and Resolution of Multiple Bands in PGT5/6.

<p>Flavonoids are a class of plant secondary metabolites that fulfill many functions <em>in planta</em> and also benefit human health. Glucosylation of flavonoids, catalyzed by glucosyltransferases (GTs), help flavonoids perform their important roles. <em>Citrus paradisi</em> (grapefruit) is known to be active in flavonoid metabolism and possesses a variety of secondary product GTs. This research is designed to test the hypothesis that PGT10 is a flavonoid glucosyltransferase. PGT10 was cloned, expressed, purified, and tested for GT activity with a variety of phenolic substrates. Efforts to identify the reaction catalyzed by PGT10 continue. A second project was designed to test the hypothesis that multiple bands obtained by RT-PCR of PGT 5/6 found in root cDNA represented a set of homologous genes. Hypothesis has been confirmed by the homologous fragments found. Additional structural information is presented.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - restricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2011

Author dc:creator

• Lin, Zhangfan

• Flavonoids
• Citrus paradisi
• Glucosyltransferase
• PSPG Box
• Clone
• Life Sciences
• Plant Biology
• Plant Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/1284

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-2475