Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 27 for “"grapefruit"”.
-
The grapefruit flavonoid naringenin as a Hepatitis C virus therapy : efficacy, mechanism and delivery
Hepatitis C virus (HCV) infection accounts for approximately 40% of chronic liver disease in the United States and results in an estimated 8,000-10,000 deaths annually. Simulations suggest that in the next decade morbidity and mortality associated with HCV infections will result in approximately …
-
Flavonoid Glucosyltranferases: Cloning and Sequencing of Putative Glucosyltranferases from <em>Citrus paradisi</em> (Grapefruit) Leaves.
… substrates by glucosyltransferases (GTs). Grapefruit contains 5 different glucosyltransferases that demonstrate differences in not only substrate but also position specificity. Previous research obtained 3 putative 5’ grapefruit GT clones using SMART RACE RT-PCR with a degenerate gene …
-
Cloning, Expression, and Biochemical Characterization of Recombinant Putative Glucosyltransferases Clone 3 and 8 from Grapefruit (Citrus paradisi)
<p>The grapefruit plant, <em>Citrus paradisi</em>, tends to accumulate high levels of flavonoid glycosides such as flavanones and flavones. Flavonoids have a vast array of important functions in plants and also in humans. Glucosyltransferases (GTs) are enzymes responsible for glucosylation …
-
cDNA Cloning, Expression and Characterization of a Putative Glucosyltransferase (GT) from Grapefruit (<em>Citrus paradisi</em>) Leaves.
… metabolites that are integral to our lives. Grapefruits are well-known for production of unique glucosylated products and the enzymes responsible are UDP-glucose:glucosyltransferases (GTs). The objective of this research was to obtain full-length clones of putative grapefruit GTs, express …
-
Cloning and Sequencing of Glucosyltransferase (GT) Genes from Grapefruit (<em>Citrus paradisi</em>) Seedlings, Searching for 7-O-GT.
… roles in plant life cycles and in human welfare. Grapefruit plants produce several glycosylated flavonoids and the glycosylation reactions are catalyzed by UDP-glucose: glucosyltransferases (GTs). Our objective was to use the SMART RACE RT-PCR strategy to obtain cDNA sequences of putative …
-
Expression and Biochemical Function of Putative Flavonoid GT Clones from Grapefruit and Identification of New Clones using the harvEST Database.
… enrichment, and biochemical characterization of grapefruit putative GT protein, PGT2, and to test the hypothesis that PGT2 is a flavonoid GT. Results showed detectable amounts of activity with quercetin, a flavonol; however, activity was lower than what would be expected if this enzyme were a …
-
Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)
… in most part exist in the glucosylated form. Grapefruit accumulates high levels of glucosylated flavonoids. Plant secondary product glucosyltransferases (GTs) catalyze the glucosylation reaction, but due to low homology at both the nucleotide and amino acid sequence level of different GTs, it …
-
Determining Putative Secondary Product Glucosyltransferase Expression During <em>Citrus paradisi</em> Growth and Development.
… kinds of flavonoid GTs exist in the tissues of grapefruit making it a model plant for studying their structure and function. The goal of this investigation is to determine the expression patterns of 7 putative secondary product GTs during grapefruit growth and development by quantifying mRNA …
-
Investigating physiological and genetic characteristics of community acquired infections and potential antimicrobial interventions
… of PVL CA MSSA and CA MRSA MW2 biofilms with grapefruit EO. The S. aureus isolates all formed biofilms and had similar molecular characteristics however one isolate (CA MRSA SR) was multidrug resistant and PVL negative. The VRE isolate was negative for biofilm formation. In suspension, …
-
Investigating the modulation of oral drug absorption using in vitro models
… cells. The clinically relevant buffering of grapefruit juice to pH 7 led to a four-fold increase in intracellular calcein and hence significant inhibition of p-glycoprotein. Buffered orange and lemon juices had no effect on the assay. Flavone derivatives had previously been found to be …
-
Modulation of drug transport by citrus fruit juices
… pattern and taxonomy of the citrus fruits. Grapefruit and pummelo are classified under the neohesperidosyl species based on dominant flavonoid pattern, while lime and lemon belong to the rutinosyl species. Classification of these fruits based on taxonomy yielded parallel groupings. Orange …
-
Fabrication of Biomass-Polymeric Adsorbent for the Removal of Selected Metal Species, Nutrients and Pathogens in Aqueous Solutions
… was fabricated by incorporating biomass (grapefruit peels) and algae (diatom) on a polymeric network for improved overall properties towards the selective toxic chemical and microbial pollutants. The structural and morphological properties of the various adsorbents were done by FTIR which …
-
Expression and Characterization of Putative Plant Secondary Product Glucosyltransferase Clone 10 from <em>Citrus paradisi</em> and Resolution of Multiple Bands in PGT5/6.
… their important roles. <em>Citrus paradisi</em> (grapefruit) is known to be active in flavonoid metabolism and possesses a variety of secondary product GTs. This research is designed to test the hypothesis that PGT10 is a flavonoid glucosyltransferase. PGT10 was cloned, expressed, purified, and …
-
Shifts in suitability distribution of deciduous and citrus fruit trees under climate change in South Africa: a large-scale empirical study
… of 9 crops (oranges, lemons, mandarins, grapefruit, apples, pears, peaches, plums, and table grapes) over South Africa in each of these scenarios. Ecocrop is an empirical model, suited to this large-scale analysis despite known and acceptable point scale inaccuracies, and driven by …
-
DEVELOPMENT OF BIO-ACTIVE FOOD PACKAGING FILMS
… (PCL) with chitosan as carrier for grapefruit seed extract (GSE). The influence of molecular weight (MW) of chitosan on the blend was investigated. Lower MW chitosan accelerated the release of GSE that led to significant improvement in plasticization, hydrophilicity and inhibition of …
-
Effects of Chemical and Enzymatic Modifications on Starch and Naringenin Complexation
<p>Naringenin is a flavanone naturally present in grapefruit and tomato skin, which has been demonstrated to have health benefits. However, because of the low water solubility and bioavailability, naringenin applications are limited. Starch inclusion complexes have been shown to improve the …
-
Effect of Genetic Variation on Salt, Sweet, Fat and Bitter Taste
… was associated with naringin taste and both grapefruit intake and preference. In the putative fat taste receptor CD36 the rs1761667 and rs1984112 SNPs were associated with intake of total, polyunsaturated and monounsaturated fats as well as oleic acid taste. Conclusions: Our findings …
-
Flavonoid Intake and Prostate Cancer Aggressiveness In African Americans and European Americans
… than age 65 and in current smokers. Orange and grapefruit juice and tea were the main contributors for total flavonoid intake. These results suggest that younger men, current smokers, and AA men may benefit the most from increased flavonoid intake.</p>
-
Sensory Research on Ginseng Food Products
… ginseng extract intensified alcohol bitterness, grapefruit pith bitterness, and medicinal bitterness of caffeine, cyclo (L-Pro-L-Val), and theobromine solutions as well as a model solution simulating milk chocolate bitterness. A model solution simulating dark chocolate bitterness showed a …
Page 1 of 2