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Showing 1 to 20 of 26 for “"Glucosyltransferase"”.
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Orally Induced Tolerance to Streptococcus Mutans and Glucosyltransferase in Neonatal Mice
Three antigens, glucosyltransferase (GTase), formalin killed Streptococcus mutans cells (vaccine) and bovine serum albumin (BSA), were used to orally induce tolerance in neonatal C57BL mice. Radial immunodiffusion was used to measure the antibody response to vaccine and BSA, and an enzyme …
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Determining Putative Secondary Product Glucosyltransferase Expression During <em>Citrus paradisi</em> Growth and Development.
… roles in plant defense and human nutrition. Glucosyltransferases (GTs) transfer sugars from high energy sugar donors to other substrates. Several different kinds of flavonoid GTs exist in the tissues of grapefruit making it a model plant for studying their structure and function. The goal of …
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cDNA Cloning, Expression and Characterization of a Putative Glucosyltransferase (GT) from Grapefruit (<em>Citrus paradisi</em>) Leaves.
… and the enzymes responsible are UDP-glucose:glucosyltransferases (GTs). The objective of this research was to obtain full-length clones of putative grapefruit GTs, express them, and characterize them. Previously, gene specific primers (from conserved PSPG box) and clone specific primers (from …
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Cloning and Sequencing of Glucosyltransferase (GT) Genes from Grapefruit (<em>Citrus paradisi</em>) Seedlings, Searching for 7-O-GT.
… reactions are catalyzed by UDP-glucose: glucosyltransferases (GTs). Our objective was to use the SMART RACE RT-PCR strategy to obtain cDNA sequences of putative grapefruit flavonoid GTs. Gene specific primers were designed from the plant secondary product glucosyltransferase (PSPG) box …
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Cloning, Heterologous Expression in Yeast, and Biochemical Characterization of Recombinant Putative Glucosyltransferase Clones 9 and 11 from Grapefruit (Citrus paradisi)
… glucosylated flavonoids. Plant secondary product glucosyltransferases (GTs) catalyze the glucosylation reaction, but due to low homology at both the nucleotide and amino acid sequence level of different GTs, it is not possible to ascribe function based on sequence only. The hypotheses that PGT …
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O antigen biosynthesis in Gram-negative bacteria: Characterization of alpha1,4-glucosyltransferase WclY from Escherichia coli O117 and development of glycosyltransferase inhibitors.
Gram-negative bacteria have a polysaccharide coat (O antigen) consisting of repeating units of oligosaccharides that is assembled by glycosyltransferases. Many of these bacteria are associated with disease, and the polysaccharides are functionally important for immune responses. In light of …
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Expression and Characterization of Putative Plant Secondary Product Glucosyltransferase Clone 10 from <em>Citrus paradisi</em> and Resolution of Multiple Bands in PGT5/6.
… Glucosylation of flavonoids, catalyzed by glucosyltransferases (GTs), help flavonoids perform their important roles. <em>Citrus paradisi</em> (grapefruit) is known to be active in flavonoid metabolism and possesses a variety of secondary product GTs. This research is designed to test the …
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Computational Approaches To Anti-Toxin Therapies And Biomarker Identification
… damage that the toxins cause. The catalytic glucosyltransferase domain of TcdA and TcdB was studied using molecular dynamics in the presence of both a protein-protein binding partner and several substrates. These experiments were combined with lead optimization techniques to create a potent …
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Cloning, Expression, and Biochemical Characterization of Recombinant Putative Glucosyltransferases Clone 3 and 8 from Grapefruit (Citrus paradisi)
… functions in plants and also in humans. Glucosyltransferases (GTs) are enzymes responsible for glucosylation reactions. In our pursuit to study the structure and function of flavonoid GTs, we have used molecular approaches to identify, clone, express, and functionally characterize the …
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Flavonoid Glucosyltranferases: Cloning and Sequencing of Putative Glucosyltranferases from <em>Citrus paradisi</em> (Grapefruit) Leaves.
… transferred to aglycone flavonoid substrates by glucosyltransferases (GTs). Grapefruit contains 5 different glucosyltransferases that demonstrate differences in not only substrate but also position specificity. Previous research obtained 3 putative 5’ grapefruit GT clones using SMART RACE RT-PCR …
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Ipsc Based Gene Correction and Disease Model of A New Class of Lgmd Due to Poglut1 Mutation
… Mutation of this enzyme leads to decreased O-glucosyltransferase activity and impaired Notch signaling, the pathways important for skeletal muscle stem cell (satellite cells) quiescence and activation. We hypothesize that reduced POGLUT1 activity and impaired Notch signaling is causative of …
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Morphologic and Transcriptomic Response to Weed Pressure in Multiple Maize (Zea mays L.) Selections and Teosinte (Zea mays L. ssp parviglumis) Lines
… and glucuronyltransferases, and quercetin glucosyltransferase) common to all selections evaluated. These common ontologies were not directly related to light depravation or quality, nutrient depravation, nor water stress, which were expected to be the primary mechanisms in weed response. …
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Elucidating The Role of Rumi and O-Glucosylation In The Drosophila Eye
<p>Rumi is a protein <em>O-</em>glucosyltransferase that adds the sugar <em>O-</em>glucose onto the serine in the target sequence C-S-X-S-(P/A)-C found within properly folded EGF repeats. It was first discovered to modify the <em>Drosophila </em>Notch extracellular domain and to be required for …
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Sclerotinia sclerotiorum : phytotoxins and metabolism of phytoalexins
… of the brassinin detoxifying enzyme (brassinin glucosyltransferase) of S.sclerotiorum was achieved.
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Biochemical and biophysical investigations of N-linked glycosylation pathways in archaea
… synthesis of Alg5, a yeast dolichyl-phosphate glucosyltransferase that shares high sequence similarity to AglK, the first glycosyltransferase in the M. voltae pathway. Dol-P-Glc was generated and examined to unambiguously characterize the stereochemistry of the product of Alg5.
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The study of shear inactivation of dextransucrase preparation from Leuconostoc mesenteroides
… dextransucrase (α 1,6-glucan: D-fructose 2-glucosyltransferase EC 2.4.1.5) was concentrated and purified from the fermentor culture of Leuconostoc mesenteroides (ATCC 10830) by a three-step procedure: centrifugation, membrane ultrafiltration and dialysis, and gel permeation chromatography. …
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Localization of monoterpenoid indole alkaloid (MIA) biosynthesis by in situ hybridization (ISH)
… novel enzymatic step, UDPG-7-deoxyloganetic acid glucosyltransferase (UGT8) to the IPAP cells of Catharanthus longifolius. These results enable the suggestion that all steps from Gl0H up to and including UGT8 occur in the IPAP cells of the leaf, making the IPAP cells the main site for the majority …
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Genetic studies of candidate genes in the glycoalkaloid biosynthetic pathway of potato
… galactosyltransferase (SGT1); and solanidine glucosyltransferase (SGT2)]. A germplasm panel of six wild potato species [Solanum chacoense (chc 80-1), S. commersonii subsp. commersonii, S. demissum, S. sparsipilum, S. spegazzinii, and S. stoloniferum] and a cultivated clone S. tuberosum Group …
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