Iron Acquisition in <em>Rhodococcus erythrolpolis</em>: the Isolation of Mutant(s) that Do Not Produce a Siderophore.

<p><em>Rhodococcus</em>, a soil bacterium, displays a diverse range of metabolic capabilities with a number of potential practical applications. To exploit the metabolic potential of <em>Rhodococcus</em>, their basic physiology, genetics, and especially the acquisition of essential nutrients like iron, must be understood.</p> <p><em>R. erythropolis</em> strain IGTS8 releases a small compound called a siderophore, that scavenges ferric iron from the environment. To learn more about the genetic control of iron acquisition, mutant(s) defective in siderophore production were isolated. Mutants were generated, by inserting a mutagenic plasmid, pJCS506, into the bacterial cell using electroporation. The plasmid, which cannot replicate in these bacterial cells, randomly inserts into the <em>R. erythropolis</em> genome producing mutations. The potential mutants were detected by screening on a chrome azurol S plate, which detects siderophore production. Colonies that failed to produce siderophore were tested by liquid assays. The strain N5-59 was confirmed as a non-siderophore producing mutant by liquid assays.</p>

Name thesis:degree_name

MS (Master of Science)

Level thesis:degree_level

Thesis - unrestricted

Discipline thesis:degree_discipline

Biology

Year dc:date.issued

2001

Author dc:creator

• Vellore, Jaishree M

• Siderophore
• Iron acquisition
• Rhodococcus
• Biology
• Life Sciences

Statement dc:rights

• Copyright by the authors.

Repository record dc:identifier

https://dc.etsu.edu/etd/41

OAI identifier oai:identifier

oai:dc.etsu.edu:etd-1091