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Eastern Michigan University
Expansion microscopy to visualize cell-cycle events in the abbreviated cell cycle in <i>Caenorhabditis elegans</i> (roundworm) germline stem cells
Abstract
dc:description.abstract<p>The abbreviated cell cycle is a poorly understood form of the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting techniques and modifications to DNA staining methods. These optimizations produced brighter samples that were easier to find and focus on at the microscope. These optimizations, combined with future labeling of DNA replication, will expand our understanding of how cells with an abbreviated cell cycle maintain genome stability.</p>
Degree
thesis:*- Name thesis:degree_name
- Master of Science (MS)
- Level thesis:degree_level
- Open Access Thesis
- Discipline thesis:degree_discipline
- Biology
- Year dc:date.available
- 2021
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Campbell, Taylor
- Contributors dc:contributor
-
- Hannah Seidel, PhD
- Aaron Liepman, PhD
- Robert Winning, PhD
Subjects
dc:subject × 2Identifiers
dc:identifier.*- Repository record dc:identifier
- https://commons.emich.edu/theses/1095
- OAI identifier oai:identifier
- oai:commons.emich.edu:theses-2456