The abbreviated cell cycle is a poorly understood form of the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting techniques and modifications to DNA staining methods. These optimizations produced brighter samples that were easier to find and focus on at the microscope. These optimizations, combined with future labeling of DNA replication, will expand our understanding of how cells with an abbreviated cell cycle maintain genome stability.
","abstract_html":"<p>The abbreviated cell cycle is a poorly understood form of the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting techniques and modifications to DNA staining methods. These optimizations produced brighter samples that were easier to find and focus on at the microscope. These optimizations, combined with future labeling of DNA replication, will expand our understanding of how cells with an abbreviated cell cycle maintain genome stability.</p>","abstract_has_math":false,"creators":["Campbell, Taylor"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Open Access Thesis","degree_discipline":"Biology","degree_department":null,"school":null,"contributors":["Hannah Seidel, PhD","Aaron Liepman, PhD","Robert Winning, PhD"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-01-01T08:00:00Z","date_published":"2021-01-01T08:00:00Z","updated_at":"2026-07-24T02:17:40Z","subjects":["Cell Biology","Other Cell and Developmental Biology"],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://commons.emich.edu/theses/1095","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Hannah Seidel, PhD","Aaron Liepman, PhD","Robert Winning, PhD"]},{"key":"dc:creator","label":"Author","values":["Campbell, Taylor"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2021-10-08T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Biology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Open Access Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Cell Biology","Other Cell and Developmental Biology"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://commons.emich.edu/theses/1095"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The abbreviated cell cycle is a poorly understood form of the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting techniques and modifications to DNA staining methods. These optimizations produced brighter samples that were easier to find and focus on at the microscope. These optimizations, combined with future labeling of DNA replication, will expand our understanding of how cells with an abbreviated cell cycle maintain genome stability.
"]},{"key":"dc:title","label":"Title","values":["Expansion microscopy to visualize cell-cycle events in the abbreviated cell cycle in Caenorhabditis elegans (roundworm) germline stem cells"]}]}],"canonical_facts":{"dc:contributor":["Hannah Seidel, PhD","Aaron Liepman, PhD","Robert Winning, PhD"],"dc:creator":["Campbell, Taylor"],"dc:date.available":["2021-10-08T07:00:00Z"],"dc:description.abstract":["The abbreviated cell cycle is a poorly understood form of the cell cycle essential for genome stability in stem cells. To visualize cell-cycle events in the abbreviated cell cycle, we optimized a protocol for expansion microscopy in stem cells of the reproductive system of the model organism C. elegans. Optimizations included development of new mounting techniques and modifications to DNA staining methods. These optimizations produced brighter samples that were easier to find and focus on at the microscope. These optimizations, combined with future labeling of DNA replication, will expand our understanding of how cells with an abbreviated cell cycle maintain genome stability.
"],"dc:identifier":["https://commons.emich.edu/theses/1095"],"dc:subject":["Cell Biology","Other Cell and Developmental Biology"],"dc:title":["Expansion microscopy to visualize cell-cycle events in the abbreviated cell cycle in Caenorhabditis elegans (roundworm) germline stem cells"],"thesis:degree_discipline":["Biology"],"thesis:degree_level":["Open Access Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T02:17:40Z"}