Chromatographic Studies Of Some Hormone And Metabolic Modulators Used For Doping In Sports

<p>The World Anti-Doping Agency (WADA) is an international agency that monitors the use of Performance Enhancing Drugs (PEDs) in sports; their mission is “to lead a collaborative worldwide movement for doping-free sport” (Who We Are, 2022). For this mission, the WADA needs quick, sensitive, reproducible, and accurate methods for analyzing athletes’ biological fluids for possible PEDs. This research focuses on developing and validating simple, sensitive, rapid, and reliable gas chromatography- mass spectrometry (GC-MS) methods for the analysis of two hormone and metabolic modulators, toremifene and trimetazidine. Previously, there has been limited GC-MS methods used to analyze toremifene and trimetazidine. Quantification of these two PEDs was based on the peak area instrument response. The performance of the proposed methods was validated for linearity, accuracy, precision, sensitivity, and limits of detection (LOD) and quantification (LOQ) according to the International Council on Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines. Furthermore, the developed method was applied for the determination of trimetazidine in urine using liquid-liquid extraction with a 37% extraction efficiency. </p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Master's

Discipline thesis:degree_discipline

Chemistry

Grantor dc:publisher

Eastern Kentucky University

Year

2022

Author dc:creator

• Speidel, Ruth Marie

• Chromatography
• Doping
• Gas Chromatography Mass Spectrometry
• Toremifene
• Trimetazidine
• Analytical Chemistry
• Toxicology

Statement dc:rights

• Copyright 2022 Ruth Marie Speidel

Repository record dc:identifier

https://encompass.eku.edu/etd/763

OAI identifier oai:identifier

oai:encompass.eku.edu:etd-1761