Effect of Melatonin on Differentiation of Human Mesenchymal Stem Cells and a Study on C-Terminal Domains of MT1 and MT2 Melatonin Receptors

Melatonin has been reported to enhance the differentiation of osteoblasts. The purpose of this study was to determine the melatonin treatment that would differentiate human mesenchymal stem cells (hAMSCs) into osteoblasts. A 21 d continuous melatonin treatment significantly increased the alkaline phosphatase (ALP) activity and the deposition of calcium in hAMSCs. These effects were inhibited by MT2 specific antagonist- 4P-PDOT. The time periods of melatonin treatment that increased the expression of osteogenic genes indicated both a sensitized and desensitized receptors with respect to cAMP signaling, signifying two distinct mechanisms of melatonin's action. Unlike the parathyroid hormone which is administered in intermittent doses to increase bone mass, a continuous melatonin treatment may be effective in having an anabolic effect on bone.

Name thesis:degree_name

PhD

Level thesis:degree_level

Immediate Access

Discipline thesis:degree_discipline

Pharmacology

Year dc:date.available

2010

Author dc:creator

• Sethi, Shalini

Contributors dc:contributor

• Paula Witt-Enderby
• Vicki Davis
• Jane Cavanaugh
• John Pollock
• Rehana Leak

• Beta-arrestin
• Desensitization
• Melatonin receptors
• Mesenchymal stem cells
• Mutations
• Osteoblasts

Language dc:language

English

Repository record dc:identifier

https://dsc.duq.edu/etd/1173

OAI identifier oai:identifier

oai:dsc.duq.edu:etd-2189