{"id":{"repo_id":"duquesne","oai_identifier":"oai:dsc.duq.edu:etd-2189"},"canonical_url":"https://search.dev.ndltd.org/etd/duquesne/oai:dsc.duq.edu:etd-2189","repository":{"repo_id":"duquesne","name":"Duquesne","base_url":"https://dsc.duq.edu/do/oai/"},"display":{"title":"Effect of Melatonin on Differentiation of Human Mesenchymal Stem Cells and a Study on C-Terminal Domains of MT1 and MT2 Melatonin Receptors","abstract":"Melatonin has been reported to enhance the differentiation of osteoblasts. The purpose of this study was to determine the melatonin treatment that would differentiate human mesenchymal stem cells (hAMSCs) into osteoblasts. A 21 d continuous melatonin treatment significantly increased the alkaline phosphatase (ALP) activity and the deposition of calcium in hAMSCs. These effects were inhibited by MT2 specific antagonist- 4P-PDOT. The time periods of melatonin treatment that increased the expression of osteogenic genes indicated both a sensitized and desensitized receptors with respect to cAMP signaling, signifying two distinct mechanisms of melatonin's action. Unlike the parathyroid hormone which is administered in intermittent doses to increase bone mass, a continuous melatonin treatment may be effective in having an anabolic effect on bone.","abstract_html":"Melatonin has been reported to enhance the differentiation of osteoblasts. The purpose of this study was to determine the melatonin treatment that would differentiate human mesenchymal stem cells (hAMSCs) into osteoblasts. A 21 d continuous melatonin treatment significantly increased the alkaline phosphatase (ALP) activity and the deposition of calcium in hAMSCs. These effects were inhibited by MT2 specific antagonist- 4P-PDOT. The time periods of melatonin treatment that increased the expression of osteogenic genes indicated both a sensitized and desensitized receptors with respect to cAMP signaling, signifying two distinct mechanisms of melatonin's action. Unlike the parathyroid hormone which is administered in intermittent doses to increase bone mass, a continuous melatonin treatment may be effective in having an anabolic effect on bone.","abstract_has_math":false,"creators":["Sethi, Shalini"],"institution":null,"degree_name":"PhD","degree_level":"Immediate Access","degree_discipline":"Pharmacology","degree_department":null,"school":null,"contributors":["Paula Witt-Enderby","Vicki Davis","Jane Cavanaugh","John Pollock","Rehana Leak"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2010,"date_issued":"2010-01-01T08:00:00Z","date_published":"2010-01-01T08:00:00Z","updated_at":"2026-07-24T02:10:29Z","subjects":["Beta-arrestin","Desensitization","Melatonin receptors","Mesenchymal stem cells","Mutations","Osteoblasts"],"languages":["English"],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dsc.duq.edu/etd/1173","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Paula Witt-Enderby","Vicki Davis","Jane Cavanaugh","John Pollock","Rehana Leak"]},{"key":"dc:creator","label":"Author","values":["Sethi, Shalini"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date.available","label":"Dc Date Available","values":["2018-03-28T07:00:00Z"]},{"key":"thesis:degree_discipline","label":"Discipline","values":["Pharmacology"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Immediate Access"]},{"key":"thesis:degree_name","label":"Degree Name","values":["PhD"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["Beta-arrestin","Desensitization","Melatonin receptors","Mesenchymal stem cells","Mutations","Osteoblasts"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["English"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dsc.duq.edu/etd/1173"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["Melatonin has been reported to enhance the differentiation of osteoblasts. The purpose of this study was to determine the melatonin treatment that would differentiate human mesenchymal stem cells (hAMSCs) into osteoblasts. A 21 d continuous melatonin treatment significantly increased the alkaline phosphatase (ALP) activity and the deposition of calcium in hAMSCs. These effects were inhibited by MT2 specific antagonist- 4P-PDOT. The time periods of melatonin treatment that increased the expression of osteogenic genes indicated both a sensitized and desensitized receptors with respect to cAMP signaling, signifying two distinct mechanisms of melatonin's action. Unlike the parathyroid hormone which is administered in intermittent doses to increase bone mass, a continuous melatonin treatment may be effective in having an anabolic effect on bone."]},{"key":"dc:title","label":"Title","values":["Effect of Melatonin on Differentiation of Human Mesenchymal Stem Cells and a Study on C-Terminal Domains of MT1 and MT2 Melatonin Receptors"]}]}],"canonical_facts":{"dc:contributor":["Paula Witt-Enderby","Vicki Davis","Jane Cavanaugh","John Pollock","Rehana Leak"],"dc:creator":["Sethi, Shalini"],"dc:date.available":["2018-03-28T07:00:00Z"],"dc:description.abstract":["Melatonin has been reported to enhance the differentiation of osteoblasts. The purpose of this study was to determine the melatonin treatment that would differentiate human mesenchymal stem cells (hAMSCs) into osteoblasts. A 21 d continuous melatonin treatment significantly increased the alkaline phosphatase (ALP) activity and the deposition of calcium in hAMSCs. These effects were inhibited by MT2 specific antagonist- 4P-PDOT. The time periods of melatonin treatment that increased the expression of osteogenic genes indicated both a sensitized and desensitized receptors with respect to cAMP signaling, signifying two distinct mechanisms of melatonin's action. Unlike the parathyroid hormone which is administered in intermittent doses to increase bone mass, a continuous melatonin treatment may be effective in having an anabolic effect on bone."],"dc:identifier":["https://dsc.duq.edu/etd/1173"],"dc:language":["English"],"dc:subject":["Beta-arrestin","Desensitization","Melatonin receptors","Mesenchymal stem cells","Mutations","Osteoblasts"],"dc:title":["Effect of Melatonin on Differentiation of Human Mesenchymal Stem Cells and a Study on C-Terminal Domains of MT1 and MT2 Melatonin Receptors"],"thesis:degree_discipline":["Pharmacology"],"thesis:degree_level":["Immediate Access"],"thesis:degree_name":["PhD"]},"updated_at":"2026-07-24T02:10:29Z"}