Replicación cromosómica en presencia de una Nucleósidodifosfato reductasa codificada por el alelo nrda101 de Escherichia coli

The nrdA101 mutant allele codes for an NDP reductase (the only specific enzyme required for the enzymatic synthesis of the precursors of DNA) which is inactivated in 2 minutes at 42°C in vitro. However, an nrdA101 mutant strain is able to synthesize DNA at 42ºC during 150 minutes in presence of rifampicin. In this work we have determined the cause of this synthesis at restrictive temperature and we have proved that NDP reductase is degraded by a protease synthesized at 42°C in the nrdA101 mutant. The results obtained suggest that NDP reductase is included in the replication hyperstructure.

Grantor dc:publisher

Universidad de Extremadura (España)

Year dc:date

2007

Author dc:creator

• Salguero Corbacho, Israel

Contributors dc:contributor

• Guzmán Cabañas, María Elena (Universidad de Extremadura)
• Jiménez Sánchez, Alfonso (Universidad de Extremadura)

Statement dc:rights

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Language dc:language

spa

Repository record dc:identifier

https://dialnet.unirioja.es/servlet/oaites?codigo=1527

OAI identifier oai:identifier

oai:dialnet.unirioja.es:TES0000000961