{"id":{"repo_id":"dialnet","oai_identifier":"oai:dialnet.unirioja.es:TES0000000961"},"canonical_url":"https://search.dev.ndltd.org/etd/dialnet/oai:dialnet.unirioja.es:TES0000000961","repository":{"repo_id":"dialnet","name":"Dialnet","base_url":"https://dialnet.unirioja.es/oaites/OAIHandler"},"display":{"title":"Replicación cromosómica en presencia de una Nucleósidodifosfato reductasa codificada por el alelo nrda101 de Escherichia coli","abstract":"The nrdA101 mutant allele codes for an NDP reductase (the only specific enzyme required for the enzymatic synthesis of the precursors of DNA) which is inactivated in 2 minutes at 42°C in vitro. However, an nrdA101 mutant strain is able to synthesize DNA at 42ºC during 150 minutes in presence of rifampicin. In this work we have determined the cause of this synthesis at restrictive temperature and we have proved that NDP reductase is degraded by a protease synthesized at 42°C in the nrdA101 mutant. The results obtained suggest that NDP reductase is included in the replication hyperstructure.","abstract_html":"The nrdA101 mutant allele codes for an NDP reductase (the only specific enzyme required for the enzymatic synthesis of the precursors of DNA) which is inactivated in 2 minutes at 42°C in vitro. However, an nrdA101 mutant strain is able to synthesize DNA at 42ºC during 150 minutes in presence of rifampicin. In this work we have determined the cause of this synthesis at restrictive temperature and we have proved that NDP reductase is degraded by a protease synthesized at 42°C in the nrdA101 mutant. The results obtained suggest that NDP reductase is included in the replication hyperstructure.","abstract_has_math":false,"creators":["Salguero Corbacho, Israel"],"institution":"Universidad de Extremadura (España)","degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["Guzmán Cabañas, María Elena (Universidad de Extremadura)","Jiménez Sánchez, Alfonso (Universidad de Extremadura)"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2007,"date_issued":"2007","date_published":"2007","updated_at":"2026-07-24T06:26:35Z","subjects":[],"languages":["spa"],"rights":["LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI"],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://dialnet.unirioja.es/servlet/oaites?codigo=1527","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Guzmán Cabañas, María Elena (Universidad de Extremadura)","Jiménez Sánchez, Alfonso (Universidad de Extremadura)"]},{"key":"dc:creator","label":"Author","values":["Salguero Corbacho, Israel"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2007"]},{"key":"dc:publisher","label":"Institution","values":["Universidad de Extremadura (España)"]},{"key":"dc:type","label":"Dc Type","values":["text (thesis)"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["spa"]},{"key":"dc:rights","label":"Dc Rights","values":["LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://dialnet.unirioja.es/servlet/oaites?codigo=1527"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["The nrdA101 mutant allele codes for an NDP reductase (the only specific enzyme required for the enzymatic synthesis of the precursors of DNA) which is inactivated in 2 minutes at 42°C in vitro. However, an nrdA101 mutant strain is able to synthesize DNA at 42ºC during 150 minutes in presence of rifampicin. In this work we have determined the cause of this synthesis at restrictive temperature and we have proved that NDP reductase is degraded by a protease synthesized at 42°C in the nrdA101 mutant. The results obtained suggest that NDP reductase is included in the replication hyperstructure.","El alelo mutante nrdA101 codifica una NDP reductasa (única enzima requerida directamente para la biosíntesis de los precursores del DNA) que in vitro se inactiva en 2 minutos a 42°C. Sin embargo, un mutante nrdA101 tiene capacidad para sintetizar DNA a 42ºC en presencia de rifampicina durante 150 minutos. En este trabajo se ha determinado la causa de esta síntesis de DNA a temperatura restrictiva y se ha demostrado que la NDP reductasa es degradada por una proteasa sintetizada a 42°C en el mutante nrdA101. Los resultados obtenidos sugieren que la NDP reductasa se encuentra incluida en la hiperestructura de replicación."]},{"key":"dc:format","label":"Dc Format","values":["application/pdf"]},{"key":"dc:title","label":"Title","values":["Replicación cromosómica en presencia de una Nucleósidodifosfato reductasa codificada por el alelo nrda101 de Escherichia coli"]}]}],"canonical_facts":{"dc:contributor":["Guzmán Cabañas, María Elena (Universidad de Extremadura)","Jiménez Sánchez, Alfonso (Universidad de Extremadura)"],"dc:creator":["Salguero Corbacho, Israel"],"dc:date":["2007"],"dc:description":["The nrdA101 mutant allele codes for an NDP reductase (the only specific enzyme required for the enzymatic synthesis of the precursors of DNA) which is inactivated in 2 minutes at 42°C in vitro. However, an nrdA101 mutant strain is able to synthesize DNA at 42ºC during 150 minutes in presence of rifampicin. In this work we have determined the cause of this synthesis at restrictive temperature and we have proved that NDP reductase is degraded by a protease synthesized at 42°C in the nrdA101 mutant. The results obtained suggest that NDP reductase is included in the replication hyperstructure.","El alelo mutante nrdA101 codifica una NDP reductasa (única enzima requerida directamente para la biosíntesis de los precursores del DNA) que in vitro se inactiva en 2 minutos a 42°C. Sin embargo, un mutante nrdA101 tiene capacidad para sintetizar DNA a 42ºC en presencia de rifampicina durante 150 minutos. En este trabajo se ha determinado la causa de esta síntesis de DNA a temperatura restrictiva y se ha demostrado que la NDP reductasa es degradada por una proteasa sintetizada a 42°C en el mutante nrdA101. Los resultados obtenidos sugieren que la NDP reductasa se encuentra incluida en la hiperestructura de replicación."],"dc:format":["application/pdf"],"dc:identifier":["https://dialnet.unirioja.es/servlet/oaites?codigo=1527"],"dc:language":["spa"],"dc:publisher":["Universidad de Extremadura (España)"],"dc:rights":["LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI"],"dc:title":["Replicación cromosómica en presencia de una Nucleósidodifosfato reductasa codificada por el alelo nrda101 de Escherichia coli"],"dc:type":["text (thesis)"]},"updated_at":"2026-07-24T06:26:35Z"}