Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 25 for “"Enzymatic Synthesis"”.
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Mechanistic Studies of the Enzymatic Synthesis of Polyesters
… information gained from these studies led to the synthesis of several novel polyesters and polyurethanes, which may have commercial utility in coatings and adhesives. We have investigated the secondary structure of the Candida antarctica lipase B enzyme using conventional CD and synchrotron …
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Towards Enzymatic Synthesis of Uncharged Xeno Nucleic Acids
… I aim to develop strategies that enable enzymatic synthesis of these uncharged XNAs. I seek to engineer polymerases to accept the requisite non-natural nucleotide substrates, thereby enabling their enzymatic synthesis and in vitro evolution. To identify polymerase mutants with improved …
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The chemical and enzymatic synthesis of poly(ADP-ribose)
Poly(ADP-ribose) synthesis and degradation are important cellular processes associated with the DNA damage response and many other pathways. Traditionally, these processes have been challenging to study due to the heterogeneity associated with the synthesis of poly(ADP-ribose). Discussed here are …
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Chemical and Enzymatic Synthesis of Dehydropeptides and Their Fluoro Analogs
… and their fluoro analogs were prepared in an enzymatic fashion. For instance, fluorinated dipeptides, L-alanine-D-fluoroalanine and L-alanine- D,L-difluoroalanine were treated with the epimerase YcjG to produce L-Ala-Dha and L-Ala-Dha(F), respectively, as determined by 1H NMR, 19F NMR and MS …
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Control of product molecular weight in the enzymatic synthesis of a biodegradable polyester, polyhydroxybutyrate
Polyhydroxybutyrate (PHB) is a polymer accumulated by many bacterial species to store carbon and reducing equivalents. PHB is packaged intracellularly in inclusions termed "granules" that contain the polymer in an amorphous state, surrounded by a protein coat. The molecular weight (Mw) of PHB is …
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Enzymatic synthesis of defined-length poly(ADP-ribose) and the investigation of cell-permeable poly(ADP-ribose) glycohydrolase inhibitors
… process. To tackle these problems, a controlled enzymatic synthesis pathway of PAR has been investigated through the use of masked β-NAD+ derivatives that can homogenously and monomerically modify PARP. In order to verify its ability to modify PARP, a sample of proparagyl-β-NAD+ derivative was …
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Synthesis and evaluation of vegetable (soybean) oil based epoxy resin for beverage can coating and enzymatic synthesis and characterization of tailored N&C end protected oligopeptides
… The research in the first part describes the synthesis and evaluation of an epoxidized ally soyate (EAS) based epoxy resin for can coating. The coating obtained with the resin was subjected to rigorous mechanical tests specific for beverage can coatings. In addition the beverage exposed to the …
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Implicaciones de la NDP reductasa en la progresión de las horquillas de replicación en Escherichia coli
… is the only specific enzyme required for the enzymatic synthesis of the precursors of DNA and it is a part of the Nucleotides Synthesis Complex. In this work we have analysed the replication fork progression in a nrdA101 mutant, which has a thermosensitive NDP reductase. We have proved that …
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Replicación cromosómica en presencia de una Nucleósidodifosfato reductasa codificada por el alelo nrda101 de Escherichia coli
… (the only specific enzyme required for the enzymatic synthesis of the precursors of DNA) which is inactivated in 2 minutes at 42°C in vitro. However, an nrdA101 mutant strain is able to synthesize DNA at 42ºC during 150 minutes in presence of rifampicin. In this work we have determined the …
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Part1. Relative antioxidant efficiency of synthetic and natural compounds; Part 2. Synthesis, characterization of cysteine oligopeptides and assessment of their antioxidant activity
… second part of the research involved enzymatic synthesis and liquid chromatographic - mass spectrometric (LC-MS) characterization of cysteine containing hydrophobic oligopeptides. Two types of oligopeptides L-cysteine homo-oligopeptides and L-cysteine, L-methionine hetero-oligopeptides …
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Enzymatic Polymerization of High Molecular Weight DNA
… capabilities. Hence, the efficient and precise synthesis of high molecular weight DNA materials has become key to advance DNA bionanotechnology. Current synthesis methods largely rely on either solid phase chemical synthesis or template-dependent polymerase amplification. The inherent …
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High throughput single molecule in situ-verified nucleic acid synthesis
… current practice, building DNA, by de novo DNA synthesis and related methods, is a rate limiting and costly bottleneck for researchers. State of the art de novo DNA Synthesis technologies, are trial-and-error, nondeterministic processes where turnaround times for specified DNA range on the order …
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NATURAL POLYPHENOLS AS LEAD COMPOUNDS IN THE SYNTHESIS OF ANTITUMOR AGENTS AND OTHER USEFUL PRODUCTS
NATURAL POLYPHENOLS AS LEAD COMPOUNDS IN THE SYNTHESIS OF ANTITUMOR AGENTS AND OTHER USEFUL PRODUCTS Abstract Some polyphenol dimers, such as lignans, neolignans and stilbenoid lignans, because of their biological properties as well as their structural variety, are an attractive target for chemical …
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Investigating asparagine-linked glycosylation substrate : specificity and effects on protein folding
… N-linked glycosylation machinery, a chemo-enzymatic synthesis for each of the glycan intermediates within this pathway was developed, which are impractical to obtain from the host organism. Importantly, chemo-enzymatic allowed for the incorporation of structural modifications for …
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An exploration of phosphorylases for the synthesis of carbohydrate polymers
… role in metabolism and their use in the in vitro synthesis of carbohydrates. The disaccharide phosphorylases have attracted attention because of their strict stereo- and regiospecificity and their tractability. The polymerising phosphorylases have received less attention due to heterogeneous …
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Encoded synthesis and evolution of clinically approved 2'-modified ribonucleic acids
… However, conventional phosphor-amidite synthesis for these 2'-modified nucleic acids is limited in length, prevents parallel exploration of different sequences and modifications, and precludes evolution. This study aims to develop an encoded enzymatic synthesis approach using polymerase …
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Development of New Photocrosslinking Approaches to Discover Binding Partners of O-GlcNAc-Modified Proteins
… Finally, I initiated the development of a chemo-enzymatic synthesis of O-GlcNDAz-ylated peptides using three enzymes: A bifunctional enzyme consisting of Bifidobacterium longum N-acetylhexosamine 1-kinase (NahK) and E. coli N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) to generate …
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