Recombinant Expression of Preptin Analogs for Alanine Scanning Mutagenesis of Residues 27-29

<p>The novel peptide preptin consists of 34 amino acid residues corresponding to Asp⁶⁹-Leu¹⁰² of proinsulin-like growth factor E-peptide. Preptin has been isolated from pancreatic beta cells, and it has been found to exhibit both metabolic and mitogenic activities. This makes preptin an attractive candidate to treat insulin-independent diabetes and osteoporosis. Alanine scanning mutagenesis was utilized to substitute an alanine for residues Trp²⁷, Arg²⁸, and Gln²⁹ of preptin in order to ultimately study the structure-activity relationship of these residues to preptin’s metabolic activity.</p>

Name thesis:degree_name

Chemistry

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Chemistry

Year dc:date.available

2020

Author dc:creator

• Anguilm, Tehgan N

Contributors dc:contributor

• Dr. Jonathan Meyers
• Dr. Daniel Holley
• Dr. Cindy Ticknor

• Preptin Analogs
• Alanine Scanning Mutagensis
• Residues 27-19
• Osteoporosis
• Metabolic Activity
• Diabetes
• Chemistry
• Organic Chemistry

Language dc:language

English

Repository record dc:identifier

https://csuepress.columbusstate.edu/theses_dissertations/390

OAI identifier oai:identifier

oai:csuepress.columbusstate.edu:theses_dissertations-1390