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Showing 1 to 20 of 44 for “"recombinant expression"”.
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Recombinant Expression and Assembly of Methyl Coenzyme-M reductase
… candidates for the development of heterologous expression systems. Thus, here we sought to generate such a system for the study of various ANME MCRs in the methanogen, Methanococcus maripaludis. We report the successful expression and purification of an ANME-2d MCR, marking a significant step …
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The recombinant expression and localization of TvCP2 of trichomonas vaginalis
… blot analysis, immunofluorescent staining, and recombinant expression. The experiments 5 presented here suggest that aT l-CP2 over-expressing transfectant line processes CP2 and sequesters it in cellular compartments. Previous data gives strong evidence of the secretion of cysteine protease CP4 …
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Recombinant Expression In Pichia pastoris System Of The Potassium Channel Inhibitor Peptide, Ts6.
… blocking activity in Kv1.3 has been analyzed recombinantly for Kv1.5 and Kv1.3. This toxin (Ts6) purified from Tityus serrulatus venom (a Brazilian scorpion) is composed of 40 amino acid residues, four disulfide bonds, and a molecular weight of 4.5 kDa. In the scope of this research, we used …
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Recombinant Expression of Preptin Analogs for Alanine Scanning Mutagenesis of Residues 27-29
<p>The novel peptide preptin consists of 34 amino acid residues corresponding to Asp<sup>69</sup>-Leu<sup>102</sup> of proinsulin-like growth factor E-peptide. Preptin has been isolated from pancreatic beta cells, and it has been found to exhibit both metabolic and mitogenic activities. This makes …
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Recombinant Expression, Purification, and Characterization of an HIV-1 Tat-Human Cyclin T1 Chimera
… data is a result of the notoriously difficult expression of native HIV-1 Tat caused in large part by the high cysteine count, and poor solubility of the Tat protein. This work presents a protocol for the expression and purification of a high affinity recombinant chimeric protein which includes …
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Analysis of the regulation of GIGANTEA transcript and protein accumulation in Arabidopsis and recombinant expression
… time control by biochemical characterisation of recombinantly expressed GI protein, and examination of GI protein cycling in planta. Expression and purification of a predicted ryegrass GI domain in Escherichia coli (E. coli) revealed that the protein aggregated and was unstable. Attempts were …
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Recombinant expression, purification and structural characterization of aspergillus niger ring finger domain using nuclear magnetic resonance spectroscopy
… domain was sub-cloned into a pGEX-6P-2 protein expression vector and heterologously over-expressed as a GST fusion protein, which was later purified using GST-Agarose affinity chromatography. The biophysical analysis of the purified protein using a 1D proton NMR spectroscopy data revealed the …
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Transmembrane glycoprotein gp41 of the Human Immunodeficiency Virus Type I : gene synthesis, recombinant expression and immunological characterization
HIV, the causative agent of AIDS, is one of the first retroviruses to infect humans. Controlling this epidemic is extremely challenging. Studies that focus on understanding the biology, biochemistry and structural biology of HIV as well as the interactions between viral components and new drug …
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Recombinant expression, purification and structural characterization of Saccharomyces cerevisiae RING finger domain using Nuclear Magnetic Resonance (NMR) spectroscopy
… presented in this dissertation reports on the recombinant expression and partial purification S. cerevisiae RING finger protein, as well as its biophysical and biochemical characterization. Structural predictions using the ProtParam tool on ExPASy provided information regarding the high …
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Analysis, fractionation and exploratory recombinant expression of novel eukaryotic l/D peptide isomerases and free D-amino acid racemases
Submission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2023-09-01 without embargo terms
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ENGINEERING WATER-SOLUBLE VARIANTS OF THE SINGLE-SUBUNIT OLIGOSACCHARYLTRANSFERASE
… an single-subunit enzyme that are amenable to recombinant expression in model organism including Escherichia coli. As a result, bacterial OSTs have been used as models to explore the mechanism of the N-linked glycosylation process in nature. These developments, notwithstanding, recombinant …
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Bioengineering the Expression of Active Recombinant Human Cathepsin G, Enteropeptidase, Neutrophil Elastase, and C-Reactive Protein in Yeast
… lactis were used to express several recombinant human proteins for further biochemical characterization. Two substitution variants of recombinant human enteropeptidase light chain (rhEPL) were engineered to modify the extended substrate specificity of this serine protease. Both were …
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Purification of Recombinant HIS6-GFP-Preptin Fusion Protiens
… NEB Q5 QuickChange methodology, followed by recombinant expression in Escherichia coli. The GFP-preptin fusion protein was purified using nickel affinity chromatography, followed by gel electrophoresis to confirm molecular weight and purity. The use of GFP facilitated real-time tracking of …
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Characterizing small molecular weight proteins from Latrodectus hesperus dragline and tubuliform silks
… named Egg Case Protein 3 (ECP-3). In this study, recombinant expression of ECP-3 in the pET-19b-SUMO vector was to facilitate purification and development of an immunological reagent. Using western blot analysis, we have demonstrated that ECP-3 is efficiently expressed in bacteria. We also …
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Enzymatic and analytical tools for the characterization of chondroitin sulfate and dermatan sulfate glycosaminoglycans
… the characterization of CS/DS oligosaccharides, recombinant expression and purification schemes were developed for chondroitinase AC and B from Flactobacterium heparinum. The recombinant enzymes were characterized using biochemical techniques and kinetic parameters were determined for their …
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Discovery and development of diagnostic biomarkers for human African trypanosomiasis
… identified were then cloned into E. coli for recombinant expression and purification. The recombinant proteins were then screened by ELISA against 145 patients’ sera from the WHO HAT specimen bank. Invariant Surface Glycoprotein (ISG) 65 and a soluble Variant Surface Glycoprotein (VSG) were …
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Development of metabolic pathways for the biosynthesis of hydroxyacids and lactones
… of the hydroxyvalerate product through the recombinant expression of thioesterase B (tesB). The production of 4VL was accomplished from levulinate by adding the lactonase paraoxonase I (PON1) to the P. putida hydroxyvalerate production system. Because 4VL was found to exist in a pH-dependent …
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Molecular identification of silk proteins in the gumfooted lines and attachment discs of the black widow spider, latrodectus hesperus
… silk, due to their cannibalistic nature, make recombinant expression of silk proteins a fundamental goal of spider silk research. In order to express silk fibers, cDNAs encoding the corresponding silk fiber products must first be isolated and identified. One of the first steps in gene …
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Biophysical investigations of the interaction between substrate binding domain of Hsp40 and the RING finger domain of Retinoblastoma Binding Protein 6
… binding parameters. This was followed by the recombinant expression of the proteins within two difference expression vectors and E. coli bacterial strains. The SBDHsp40 protein was purified using affinity chromatography while the RING finger domain protein was purified to homogeneity by …
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