Recombinant Expression of Preptin Analogs for Alanine Scanning Mutagenesis of Residues 27-29
The novel peptide preptin consists of 34 amino acid residues corresponding to Asp69-Leu102 of proinsulin-like growth factor E-peptide. Preptin has been isolated from pancreatic beta cells, and it has been found to exhibit both metabolic and mitogenic activities. This makes preptin an attractive candidate to treat insulin-independent diabetes and osteoporosis. Alanine scanning mutagenesis was utilized to substitute an alanine for residues Trp27, Arg28, and Gln29 of preptin in order to ultimately study the structure-activity relationship of these residues to preptin’s metabolic activity.
Preptin Analogs, Alanine Scanning Mutagensis, Residues 27-19, Osteoporosis, Metabolic Activity, Diabetes, Chemistry, Organic Chemistry
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