LC-MS/MS determination of various drugs of abuse and metabolites in municipal wastewater effluent samples

A method was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification and confirmation of 8 drugs of abuse (cocaine, codeine, MDMA, methadone, methamphetamine, morphine, nicotine, and oxycodone) and their various metabolites (acetylmorphine, cotinine, EDDP, amphetamine and benzoylecgonine) in municipal wastewater effluent samples. Samples were collected once daily and drugs were extracted from the wastewater with solid phase extraction using Waters Oasis MCX cartridges. Ultra-high-pressure liquid chromatography (UPLC) and positive electrospray ionization was used along with dynamic multiple reaction monitoring (MRM) tandem mass spectrometry for identification and quantitation. The extraction method was validated with matrix matched spiked samples at the limits of quantitation (0.5 ng/mL to 30 ng/mL) for each analyte with recoveries ranging from 70%-140%. Deuterated internal standards for each analyte were used to correct for matrix effects, ion suppression, and sample preparation errors. The validated method was applied to municipal wastewater samples collected from a point source effluent into Fossil Creek, Fort Collins, CO. Eight of thirteen drugs being measured were found on a daily basis with the maximum being 307.72 mg/min of benzoylecgonine. Samples showed various spikes in drug concentration at 7 day intervals that corresponded with weekends.

Name thesis:degree_name

Master of Science (M.S.)

Level thesis:degree_level

Masters

Discipline thesis:degree_discipline

Environmental and Radiological Health Sciences

Grantor dc:publisher

Colorado State University. Libraries

Year dc:date.issued

2012

Authors dc:creator

• McPeters, Ryley Adam, author
• Dooley, Gregory, advisor
• Hanneman, William, advisor
• Borch, Thomas, committee member

• drugs of abuse
• wastewater
• metabolites
• LC-MS/MS

Statement dc:rights

• Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.

Language dc:language.iso

eng, English

Identifier URI

https://doi.org/10.25675/3.018449

Identifier

ETDF2012400435ERHS

OAI identifier oai:identifier

oai:mountainscholar.org:10217/74426