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Ultra-high-pressure liquid chromatography (UPLC) and positive electrospray ionization was used along with dynamic multiple reaction monitoring (MRM) tandem mass spectrometry for identification and quantitation. The extraction method was validated with matrix matched spiked samples at the limits of quantitation (0.5 ng/mL to 30 ng/mL) for each analyte with recoveries ranging from 70%-140%. Deuterated internal standards for each analyte were used to correct for matrix effects, ion suppression, and sample preparation errors. The validated method was applied to municipal wastewater samples collected from a point source effluent into Fossil Creek, Fort Collins, CO. Eight of thirteen drugs being measured were found on a daily basis with the maximum being 307.72 mg/min of benzoylecgonine. 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The extraction method was validated with matrix matched spiked samples at the limits of quantitation (0.5 ng/mL to 30 ng/mL) for each analyte with recoveries ranging from 70%-140%. Deuterated internal standards for each analyte were used to correct for matrix effects, ion suppression, and sample preparation errors. The validated method was applied to municipal wastewater samples collected from a point source effluent into Fossil Creek, Fort Collins, CO. Eight of thirteen drugs being measured were found on a daily basis with the maximum being 307.72 mg/min of benzoylecgonine. Samples showed various spikes in drug concentration at 7 day intervals that corresponded with weekends.","abstract_has_math":false,"creators":["McPeters, Ryley Adam, author","Dooley, Gregory, advisor","Hanneman, William, advisor","Borch, Thomas, committee member"],"institution":"Colorado State University. 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