Assessing the Efficacy of Bacteriological Analytical Manual (BAM) Enrichment Broths for Detection of <em>Salmonella</em> spp. in Meat Analogs

<p>Plant-based meat alternatives are gaining popularity as the number of market options increases. However, the recent appearance of these products on the market post-dates the validation of the current approach for isolating <em>Salmonella</em> from meat analogs. Therefore, the objective<strong> </strong>of this study was to compare the efficacy of the currently used Bacteriological Analytical Manual (BAM) pre-enrichment broth (lactose broth) with two additional broths (universal pre-enrichment broth and buffered peptone water) for the detection of <em>Salmonella</em> in five plant-based burger products. Each burger product was spiked with a <em>Salmonella</em> serotype (<em>S.</em> Enteritidis or <em>S. </em>Agona) that has previously been linked to one or more of the protein sources in the product. The U.S. Food and Drug Administration (FDA) Matrix Extension Verification Procedure section 5.1.1 was followed for the verification of each broth. Seven replicates of each of the five selected plant-protein burgers were spot-inoculated with 30 or less colony-forming units (CFU) of <em>Salmonella enterica </em>per 25 g of product and enriched in each broth. An additional seven non-inoculated replicates were tested with all broths. Pathogen recovery was evaluated using the methods described in BAM and <em>Salmonella</em> was confirmed with real-time PCR, using two DNA templates per sample. Comparison of the number of positive real-time PCR detections for <em>Salmonella</em> across all three broths and five brands indicated no significant difference (p-value > 0.05) between the effectiveness of each broth. The greatest level of detection was found with LB, in which 95.71% of DNA templates tested positive for <em>Salmonella</em>, followed by BPW and UP broth, which each had an 88.57% detection rate. When the broths were compared for effectiveness in each product separately, LB showed a significantly greater percentage of positive detections (92.86%) in Beyond Meat^TM samples compared to UP (50%). However, none of the other products showed significant differences in detection among the three broths. In conclusion, the findings demonstrate that LB, the current BAM pre-enrichment broth, is an effective pre-enrichment for the detection of <em>Salmonella </em>in meat analogs formulated with various plant-proteins.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Food Science

Year dc:date.available

2021

Author dc:creator

• Sampson, Georgia

Contributors dc:contributor

• Rosalee Hellberg
• Anuradha Prakash
• Lilian Were

• buffered peptone water
• lactose broth
• meat-analogs
• plant-protein ingredients
• Salmonella
• universal pre-enrichment broth
• Food Microbiology
• Food Science

Repository record dc:identifier

https://digitalcommons.chapman.edu/food_science_theses/21

OAI identifier oai:identifier

oai:digitalcommons.chapman.edu:food_science_theses-1021