Microplate Immunocapture Coupled with LAMP-BART and Selective Plating for the Rapid Detection of <em>Salmonella</em> Infantis in Dry Dog Food and Treats

<p>The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of <em>Salmonella </em>in pet food with loop-mediated isothermal amplification with bioluminescent assay in real time (LAMP-BART) and selective plating on XLD. Dog food and pig ear treats were inoculated with <em>Salmonella </em>Infantis at concentrations of 100-104 CFU/25 g, followed by a 3-h enrichment, then microplate IC and LAMP-BART or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by LAMP-BART or selective plating<em>. </em>Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of <em>Salmonella </em>in 100% of dog food and treat samples tested, including at levels as low as 100 CFU/25 g. Microplate IC coupled with LAMP-BART enabled detection of <em>Salmonella </em>in dog food and treat samples down to levels of 100 CFU/25 g, with overall detection rates of 90-93%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of <em>Salmonella </em>in complex matrices when coupled with LAMP-BART or selective plating.</p>

Name thesis:degree_name

Master of Science (MS)

Level thesis:degree_level

Thesis

Discipline thesis:degree_discipline

Food Science

Year

2021

Author dc:creator

• Rosen, Danielle

Contributors dc:contributor

• Rosalee Hellberg
• Lillian Were
• Anuradha Prakash

Repository record dc:identifier

https://digitalcommons.chapman.edu/food_science_theses/18

OAI identifier oai:identifier

oai:digitalcommons.chapman.edu:food_science_theses-1018