The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of Salmonella in pet food with loop-mediated isothermal amplification with bioluminescent assay in real time (LAMP-BART) and selective plating on XLD. Dog food and pig ear treats were inoculated with Salmonella Infantis at concentrations of 100-104 CFU/25 g, followed by a 3-h enrichment, then microplate IC and LAMP-BART or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by LAMP-BART or selective plating. Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of Salmonella in 100% of dog food and treat samples tested, including at levels as low as 100 CFU/25 g. Microplate IC coupled with LAMP-BART enabled detection of Salmonella in dog food and treat samples down to levels of 100 CFU/25 g, with overall detection rates of 90-93%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of Salmonella in complex matrices when coupled with LAMP-BART or selective plating.
","abstract_html":"<p>The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of <em>Salmonella </em>in pet food with loop-mediated isothermal amplification with bioluminescent assay in real time (LAMP-BART) and selective plating on XLD. Dog food and pig ear treats were inoculated with <em>Salmonella </em>Infantis at concentrations of 100-104 CFU/25 g, followed by a 3-h enrichment, then microplate IC and LAMP-BART or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by LAMP-BART or selective plating<em>. </em>Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of <em>Salmonella </em>in 100% of dog food and treat samples tested, including at levels as low as 100 CFU/25 g. Microplate IC coupled with LAMP-BART enabled detection of <em>Salmonella </em>in dog food and treat samples down to levels of 100 CFU/25 g, with overall detection rates of 90-93%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of <em>Salmonella </em>in complex matrices when coupled with LAMP-BART or selective plating.</p>","abstract_has_math":false,"creators":["Rosen, Danielle"],"institution":null,"degree_name":"Master of Science (MS)","degree_level":"Thesis","degree_discipline":"Food Science","degree_department":null,"school":null,"contributors":["Rosalee Hellberg","Lillian Were","Anuradha Prakash"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2021,"date_issued":"2021-05-01T07:00:00Z","date_published":"2021-05-01T07:00:00Z","updated_at":"2026-07-24T01:38:16Z","subjects":[],"languages":[],"rights":[],"rights_urls":[],"identifier_entries":[]},"links":{"outbound_url":"https://digitalcommons.chapman.edu/food_science_theses/18","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["Rosalee Hellberg","Lillian Were","Anuradha Prakash"]},{"key":"dc:creator","label":"Author","values":["Rosen, Danielle"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"thesis:degree_discipline","label":"Discipline","values":["Food Science"]},{"key":"thesis:degree_level","label":"Degree Level","values":["Thesis"]},{"key":"thesis:degree_name","label":"Degree Name","values":["Master of Science (MS)"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["https://digitalcommons.chapman.edu/food_science_theses/18"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description.abstract","label":"Abstract","values":["The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of Salmonella in pet food with loop-mediated isothermal amplification with bioluminescent assay in real time (LAMP-BART) and selective plating on XLD. Dog food and pig ear treats were inoculated with Salmonella Infantis at concentrations of 100-104 CFU/25 g, followed by a 3-h enrichment, then microplate IC and LAMP-BART or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by LAMP-BART or selective plating. Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of Salmonella in 100% of dog food and treat samples tested, including at levels as low as 100 CFU/25 g. Microplate IC coupled with LAMP-BART enabled detection of Salmonella in dog food and treat samples down to levels of 100 CFU/25 g, with overall detection rates of 90-93%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of Salmonella in complex matrices when coupled with LAMP-BART or selective plating.
"]},{"key":"dc:source","label":"Dc Source","values":["Rosen, D.K. (2021). Microplate immunocapture coupled with LAMP-BART and selective plating for the rapid detection of Salmonella infantis in dry dog food and treats. Master's thesis, Chapman University. https://doi.org/10.36837/chapman.000225"]},{"key":"dc:title","label":"Title","values":["Microplate Immunocapture Coupled with LAMP-BART and Selective Plating for the Rapid Detection of Salmonella Infantis in Dry Dog Food and Treats"]}]}],"canonical_facts":{"dc:contributor":["Rosalee Hellberg","Lillian Were","Anuradha Prakash"],"dc:creator":["Rosen, Danielle"],"dc:description.abstract":["The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of Salmonella in pet food with loop-mediated isothermal amplification with bioluminescent assay in real time (LAMP-BART) and selective plating on XLD. Dog food and pig ear treats were inoculated with Salmonella Infantis at concentrations of 100-104 CFU/25 g, followed by a 3-h enrichment, then microplate IC and LAMP-BART or microplate IC and selective plating on XLD. Another set of samples underwent a traditional 24-h enrichment followed by LAMP-BART or selective plating. Based on the results of three independent trials, microplate IC followed by selective plating enabled detection of Salmonella in 100% of dog food and treat samples tested, including at levels as low as 100 CFU/25 g. Microplate IC coupled with LAMP-BART enabled detection of Salmonella in dog food and treat samples down to levels of 100 CFU/25 g, with overall detection rates of 90-93%. These results indicate high potential for microplate IC to be used in place of the traditional 24-h enrichment step, enabling detection of Salmonella in complex matrices when coupled with LAMP-BART or selective plating.
"],"dc:identifier":["https://digitalcommons.chapman.edu/food_science_theses/18"],"dc:source":["Rosen, D.K. (2021). Microplate immunocapture coupled with LAMP-BART and selective plating for the rapid detection of Salmonella infantis in dry dog food and treats. Master's thesis, Chapman University. https://doi.org/10.36837/chapman.000225"],"dc:title":["Microplate Immunocapture Coupled with LAMP-BART and Selective Plating for the Rapid Detection of Salmonella Infantis in Dry Dog Food and Treats"],"thesis:degree_discipline":["Food Science"],"thesis:degree_level":["Thesis"],"thesis:degree_name":["Master of Science (MS)"]},"updated_at":"2026-07-24T01:38:16Z"}