Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 67 for “"microplate"”.
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Distributed Feedback Laser Biosensor in Microplate Format
… incorporated into standard format 96-well microplates. A room temperature nanoreplica molding process was used to create subwavelength periodic grating structures, while a horizontal dipping process was used to apply a ~300 nm, dye-doped polymer film. In this work, the DFBLB emission …
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Development of a Microplate-Based Calcium Assay for Studying Desensitization of the P2Y2 Nucleotide Receptor
… human 1321N1 cells. To test this hypothesis, a microplate-based calcium assay was developed and employed to functionally assess P2Y2R desensitization in a high-throughput manner. The mRNA expression of GRK2 and β-arrestin-1 was knocked down using siRNA, and knockdown was verified via qRT-PCR. …
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Design and Development of a Novel Immunological Method to Quantify target-specific cysteine redox state in a microplate
… target-specificity, antibody-functionalised<br/>microplates were utilised, where IRAK-1-specific thiol oxidation could be<br/>accurately quantified in percentage terms. Using a pair-matched detector<br/>antibody enabled target protein concentration to be quantified in tandem with<br/>percentage …
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Development and Application of a Fluorescence Anisotropy Microplate Assay for Analyzing the Interaction of Steroid Hormone Receptors With DNA and Coactivators
… this interaction, we used ultra low-volume microplates to develop the simple and rapid fluorescence anisotropy microplate assay (FAMA). The FAMA was applied to the binding of estrogen and progesterone receptors to their respective DNA response elements. The FAMA is also the first system …
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Qualify Microplate Reader’s Specification and Establish the Acceptance Criteria for Modified PicoGreen Kit in Measuring cell-free DNA Concentration in Clinical Laboratory
… use of a modified PicoGreen kits and Biotek’s microplate reader to measure cfDNA concentration. This assay is being qualified to allow the laboratory to assess the yields of dsDNA after extraction method. Qualification includes performance for linearity, accuracy, precision and stability of …
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Microplate Immunocapture Coupled with LAMP-BART and Selective Plating for the Rapid Detection of <em>Salmonella</em> Infantis in Dry Dog Food and Treats
<p>The objective of this study was to use microplate immunocapture (IC) to reduce the enrichment time required for detection of <em>Salmonella </em>in pet food with loop-mediated isothermal amplification with bioluminescent assay in real time (LAMP-BART) and selective plating on XLD. Dog food and …
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Microfabricated Acoustic Sensors for the Detection of Biomolecules
… resonators are designed to operate in flexural microplate modes, with several modes shown to be ideally suited for fluid based biosensing due to improved performance in the liquid environment. The increase in quality factor of these MEMS microplate devices in liquid, as compared to air, is …
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Investigating the Kinematics and Dynamics of Strain Localization in the Northern Western Branch of the East African Rift System
… boundary between the Nubian plate and Victoria microplate, comprising magma-rich Lakes George-Edward graben in southwestern Uganda and the magma-poor Albertine and Rhino grabens in northwestern Uganda. The physics of strain localization in the northern Western Branch is not well understood. This …
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Late Neogene Uplift of the Fairweather Ground on the Basis of Bathymetric and Seismic Data from the Gulf of Alaska
… load is deposited onto the Yakutat block, a microplate that takes up the strike-slip to convergent movement with respect to North America and Pacific plates. Tectonic activity during the last 5 million years has resulted in Eocene rock exposed at the sea floor. High resolution bathymetry data …
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Seismic imaging of the lithosphere-asthenosphere boundary with a dense broadband array in central California
… plausible origins for the anomaly, the Monterey microplate (Wang et al., 2013) and Sierra Nevada batholith (Ducea & Saleeby, 1998). Results include 918 binned receiver functions that were made using multi-channel spectral deconvolution and an array-based spectral source estimation on the event …
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Enzyme-linked immunosorbent assay for red cell antibody quantitation.
… reagent red blood cells (RBC) was achieved in microplate precoated with poly-L lysine (PLL). The optimum concentrations of PLL and RBC were between 10 and 20 mg/ml and 0.5% volume for volume, respectively.<br/><br/>Experiments using serial dilutions of monoclonal anti-A, anti-B and anti-D …
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Comparative Cytotoxicity and Genotoxicity of Drinking Water Disinfection by -Products
… alone should be questioned. The in vitro microplate based bacterial and mammalian assays developed and used in this research can be employed for screening other important chemicals in the environment.
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Detection And Characterization of Insecticide Resistance Mechanisms in Culex Tarsalis
… lab-reared colony was used for comparison. Microplate assays were completed to investigate elevated levels of detoxification enzymes present as well as AChE. PCR was used to amplify the VGSC and ace-1 genes. Amplicons were sequenced and aligned to determine if mutations were present. No …
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Bioinstrumentation and Statistical Methods for Investigating Host-Microbial Interactions
… I propose a microbial co-culture assay (“porous microplate”) that spatially controls diffusion mediated metabolite exchange. Using a model host alga Phaeodactylum tricornutum, I describe bacterial responses to algal metabolites in the porous microplate. I extend the findings to provide an insight …
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Modeling and Simulation of MEMS Devices
… we introduce a reduced-order model of flexible microplates under electric excitation. The model utilizes the von Karman plate equations to account for geometric nonlinearities due to large plate deflections. The Galerkin approach is employed to reduce the partial-differential equations of motion …
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Formation and Evolution of Valdivia Bank, Walvis Ridge, South Atlantic Ocean from Geophysical Data and International Ocean Discovery Program Expedition 391 Drilling Results
… Valdivia Bank is complex, revealing a potential microplate and ridge reorganization in the Late Cretaceous, and late-stage volcanism in the Eocene. In 2019, geophysical surveys of Valdivia Bank were carried out on expedition TN-373 and TN-374 aboard the R/V Thomas G. Thompson. In 2021, the …
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THE TECTONIC EVOLUTION OF THE HATAY OPHIOLITE OF SOUTHEAST TURKEY
… existing models for the rotation of the Troodos microplate have been revised to incorporate a larger area and also account for the rotations of the Hatay and Baer-Bassit ophiolites. Restoration of sheeted dykes to their original orientations implies that a primary variation in dyke strike existed …
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An overview of the volcano-tectonic hazards of Portland, Oregon, and an assessment of emergency preparedness
… and volcanic eruptions. The young Juan de Fuca microplate is subducting under North America, introducing not only arc magmatism into the overlying plate, but also interplate and intraplate seismicity related to the subduction zone. Large crustal earthquakes are also probable in Portland because …
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Drinking water disinfection by-products: toxicological impacts and biological mechanisms induced by individual compounds or as complex mixtures
… acids (HAAs), iii) develop a single well microplate-based ATP-protein assay as a novel toxicity metric for DBPs, iv) investigate the occurrence and in vitro mammalian cell toxicity of DBPs in European drinking water samples collected from the site where epidemiological studies on …
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