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University of Central Lancashire
The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR.
Abstract
dc:description.abstractThe general aims of this research were to find an effective method for extracting DNA from bone and to use this methodology to create Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR profiles were created for several species using a number of different primers. Individuality between closely related species was also achieved. This research could go on to help in the archaeological field with identifj'ing unknown bone fragments.
Degree
thesis:*- Name dc:type.qualificationname
- msc
- Level dc:type.qualificationlevel
- doctoral
- Grantor dc:publisher.institution
- University of Central Lancashire
- Year dc:date.issued
- 2003
Author and committee
dc:creator, dc:contributor.*- Author dc:creator
-
- Ogden, Samantha
Rights
- Language dc:language
- en