The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR.
type: CreativeWork
description: The general aims of this research were to find an effective method for extracting DNA from bone and to use this methodology to create Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR profiles were created for several species using a number of different primers. Individuality between closely related species was also achieved. This research could go on to help in the archaeological field with identifj'ing unknown bone fragments.
abstract: The general aims of this research were to find an effective method for extracting DNA from bone and to use this methodology to create Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR profiles were created for several species using a number of different primers. Individuality between closely related species was also achieved. This research could go on to help in the archaeological field with identifj'ing unknown bone fragments.
author: Ogden, Samantha
language: en
date published: 2003-04
date modified: 2026-07-24T01:35:46Z
provider: University of Central Lancashire
publisher: Networked Digital Library of Theses and Dissertations
type: WebPage
source organization: University of Central Lancashire