Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 100 for “"nuclear DNA"”.
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The Functions of Human Dna2 in Mitochondrial and Nuclear DNA Maintenance
Coordination between DNA replication, DNA repair and cell-cycle progression ensures high fidelity DNA replication thus preventing mutations and DNA rearrangements. Interestingly, in addition to nuclear DNA stability, mitochondrial DNA: mtDNA) integrity is also essential for normal development. The …
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The optimisation of protocols for the extraction of nuclear DNA from animal bone forPCR.
… were to find an effective method for extracting DNA from bone and to use this methodology to create Randomly Amplified Polymorphic DNA- Polymerase Chain Reaction (RAPD-PCR) profiles of different species. A modified Chelex method was selected as the preferred extraction procedure and RAPD-PCR …
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Protein purification and cDNA cloning of suGF1 : a sea urchin nuclear DNA-binding factor
… since they may involve alterations in their DNA structure, the binding of G-string factors and in some cases even the displacement of a nucleosome positioned over this area. A poly( dG).poly( de)-binding protein (suGF1) has previously been identified and purified on a small scale from …
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The Significance of Inter- and Intraspecific DNA Content Variation in Agriculturally Important Species
This dissertation examined the total nuclear DNA content variation in agriculturally important plant species and determined any differences. The nuclear DNA content of 40 Malus species and hybrids was examined using flow cytometry. Estimates of total nuclear DNA content for this collection ranged …
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Genetic and Morphological Analyses of Three Freshwater Mussel Species in Isolated River Drainages Across Appalachia
… Green River drainages. I used the mitochondrial DNA (mtDNA) gene from the first subunit of the NADH dehydrogenase (ND1) as a genetic marker for species-level assessment of L. ortmanni and L. vanuxemensis from the Green and Cumberland River drainages. Additional mtDNA sequences of L. ortmanni and …
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A sequencing-based analyisis of epigenetic modifications of the mitochondrial genome
… the most important epigenetic modifications of nuclear DNA, where it regulates gene expression. Methylation of mitochondrial DNA (mtDNA) has been studied using whole genome bisulfite sequencing (WGBS), but recent evidence has uncovered major technical issues, which introduce a potential bias …
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Taxonomy, phylogeny and eco-biogeography of southern African white-eyes (Zosterops spp.) Aves: order Passeriformes, Family: Zosteropidae
… characters and molecular (mitochondrial and nuclear) DNA sequences. A broad scale phylogeographic analysis was also performed to establish the evolutionary process driving the diversity observed among these birds.
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Determining low levels of Africanization in unmanaged honey bee colonies using three diagnostic techniques
… With morphometrics, mitochondrial DNA, and nuclear DNA tested with the use of microsatellites we found that the known Africanized bees collected by the Florida Department of Agriculture did not exhibit Africanization other than in the preliminary, morphometric test performed by the …
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Molecular analysis of glycogen phosphorylase-1 gene expression during the development of dictyostelium discoideum
… (Rutherford, et. aI., 1991). A complementary DNA (cDNA) encoding glycogen phosphorylase-}, gp-l, was isolated from a λgtll expression library made from amoebae stage mRNA. The 5' upstream region of the gp-l gene was cloned by inverted polymerase chain reaction (IPCR) and partial genomic DNA …
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Morphometric and genetic variation among populations of the southern short-tailed shrew, Blarina carolinensis, located along the Coastal Plain of Virginia
… and molecular genetic characters (mitochondrial DNA control region and exon-primed intron-crossing nuclear DNA, EPIC) from peninsular populations of B. carolinensis and compared them to shrews of the mainland population to address this question. The sources of specimens were shrews ensnared in …
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Phylogeny reconstruction of the Schoeneae (Cyperaceae) with a focus on southern African genera
In this study both plastid and nuclear DNA sequences (rbcL, trnL-trnF, rps16, ITS and ETS) were analysed. New sequences were added to the matrix from Verboom (2006). Parsimony method was used for phylogeny reconstruction. Morphological characters were then optimised on the parsimony tree using both …
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Studies on Type I and Type II DNA Topoisomerases From Mammalian Mitochondria
<p>Mammalian mitochondria contain a unique DNA topoisomerase I with characteristics of both nuclear and bacterial type I DNA topoisomerases. This enzyme, purified from bovine liver, requires cations as cofactors and possesses optimal activity at pH 7.0, 37°C and either 150 mM K+ or Na+ ions. …
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The Influence of Nucleic Acids of Yolk Origin on the Growth and Development of the Avian Yolk Sac
… hereditary information is coded primarily into nuclear DNA, characteristic differentiated phenotypes being expressed through selective read-out of particular loci of the DNA helices. But this information has not solved the central problem of the control of development even though the Jacob and …
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Decline of the Savannah River Redeye Bass (Micropterus Coosae) Due to Introgressive Hybridization With Invasive Alabama Spotted Bass (Micropterus Punctulatus Henshalli)
… Savannah River drainage system. Three bi-allelic nuclear DNA loci and one mitochondrial DNA locus were used to examine the degree of hybridization and introgression over both temporal and spatial scales. These results are important for the management and conservation of rare fish species and adds …
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Genetic Structure of Yonahlossee Salamander Populations.
… as conservation units. One mitochondrial DNA marker as well one nuclear DNA marker were amplified using polymerase chain reaction. After analysis, both markers show genetic differentiation suggesting geographic isolation. This information can be used by management agencies for the …
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Morphometric and molecular analysis of two distinct forms of Pseudocrossidium crinitum
… 16 morphometric variables and chloroplast and nuclear DNA molecular information to determine whether Pseudocrossidium crinitum should be separated into two species based on morphological differences. We use ANOVA, Principal components analysis and cluster analysis to analyse the morphometric …
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Systematics of the spider genera Mallos and Mexitilia (Arameae: Dictynidae)
… and one species of Mexitilia mitochondrial and nuclear DNA sequences were analyzed by restriction digest. The seventeen resulting restriction sites produced a cladogram that agreed with the one based on the morphology of these six species. When morphological and molecular characters were …
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Repetitive DNA sequences in maize: Possible roles in plant adaptation and transient gene expression
Fluctuations as high as 40% in the nuclear DNA content in maize have been observed as a result of adaptive response. The knob DNA sequence has been reported to contribute 16% of this variation. Other DNA sequences involved in genome size variation due to adaptive response have yet to be identified. …
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Genetic and morphological means of differentiating within and between populations of Widdringtonia whytei and Widdringtonia nodiflora on Mount Mulanje, Malawi
… of genetic variation included PsbA (chloroplast DNA), ITS (nuclear DNA), microsatellite and ISSR gene region markers. Out of the markers that amplified successfully, it was found that there is little genetic variation present with no differentiation between or within taxonomic groups for the gene …
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Genetic and morphological diversity in the Icelandic woodland strawberry (Fragaria vesca L.)
… was to sequence genome barcodes from chloroplast DNA and nuclear DNA. The results were used to construct a phylogenetic tree using the Neighbor-Joining method. The results indicate some level of biodiversity within the Icelandic population both morphologically and genetically. The morphology …
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