An in vitro model of mouse corneal endothelial development Mohammed Mubeen Goolam.

The principle aim of this study was to derive an in vitro model that could be used to study corneal endothelial development. This was done by investigating methods to induce a mesenchymal-to-epithelial transition (MET) in mouse periocular mesenchyme cells to drive them to form fully differentiated adult corneal endothelial cells in culture. Periocular mesenchyme surrounding the optic cup was dissected from E12.5 and E13.5 mouse embryos and immortalised with the SV40 large T-antigen. Two cell lines were thus derived and named E12.5POM and E13.5POM respectively.

Grantor dc:publisher.institution

Department of Medicine

Year dc:date.issued

2012

Author dc:creator

• Goolam, Mohammed Mubeen

Advisor dc:contributor.advisor

• Kidson, S

Language dc:language.iso

eng

Handle dc:identifier.uri

http://hdl.handle.net/11427/3391

OAI identifier oai:identifier

oai:open.uct.ac.za:11427/3391