Hochauflösende multispektrale Fluoreszenzmikroskopie mit lateralen stehenden Wellen

Fluorescence microscopy, especially when considering recent new innovations, has become very important to resolve structures within biological cells. Several microscopes to improve lateral resolution in 1D and 2D have been developed by this thesis. It could be shown that an improvement of the optical resolution by a factor of two can be achieved by the new technique developed here by generating a lateral structure within the plane of the object with a freely selectable fringe distance as well as angular directions. It has been shown that excitation by total internal reflection is also possible using this construction. To characterize the instrument extensive physical measurements on beads as well as simulations were carried out. Furthermore, first succesful measurements on biological objects were made. The new microscope offering structured illumination is especially suitable for biological applications since horizontally orientated slides can be observed from below. For multispectral measurements four different excitation wavelengths are available.

Level thesis:degree_level

thesis.doctoral

Grantor dc:publisher

Universität Bielefeld

Year

2008

Author dc:creator

• Amberger, Roman

Repository record source_url

https://pub.uni-bielefeld.de/record/2303117

OAI identifier oai:identifier

oai:pub.uni-bielefeld.de:2303117