Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"Structured Illumination"”.
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Imaging nanoscale pollen morphology with Superresolution Structured Illumination Microscopy
… compared to optical microscopy. Superresolution Structured Illumination Microscopy (SR-SIM) is an emerging method that presents a powerful, non-destructive, and optically-sectioned way of imaging pollen that avoids certain disadvantages of electron microscopy. We examined and optimized the …
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Wide-field structured illumination microscopy for fluorescence and pump-probe imaging
… laser power to optimize imaging speed. Structured illumination microscopy is a super-resolution method that relies on patterned excitation. This thesis has presented novel applications of structured illumination microscopy to surface plasmon resonance fluorescence and pump-probe …
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Single-Shot Optical Sectioning Using Polarised Illumination Coded Structured Illumination Microscopy (picoSIM)
… wide-eld microscope features a uniform illumination of an extended sample region. A problem arises with this setup since light from outof-focus uorophores is also detected. The result is poor quality in the nal image, as out-of-focus structures appear blurred; furthermore, their emission …
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Theoretical and Experimental Concepts to Increase the Performance of Structured Illumination Microscopy
… implementation, and overall capabilities of structured illumination microscopy (SIM). SIM is a superresolution technique that excels in gentle live-cell volumetric imaging tasks. Many modalities of SIM were developed over the last decade that tailored SIM into the versatile and powerful …
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Beating Abbe's diffraction limit in optical microscopy via non classical photon statistics and structured illumination
L'abstract è presente nell'allegato / the abstract is in the attachment
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Extreme ultraviolet polarization optics for polarimetry of structured high harmonics
… polarimetry of harmonic light generated with structured illumination. Characterization of the fundamental light’s structured illumination and polarization profile combined with similar characterization of the harmonics can reveal insights into the HHG process. This thesis outlines steps to …
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Optimal standoff imaging using structured laser illumination and graphical models
Structured illumination can be used to form images without using a lens or a detector array. A series of spatially-structured laser pulses is cast on the scene of interest, and a single-detector power measurement is made on the light each pulse returns from the scene. There has been significant …
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Miniature Microscope Design And Construction Based On Tilted Rotationally Asymmetric Printed Lenses
… photosensitive hybrid sol-gel glass material. Illumination was delivered by high-brightness Light Emitting Diodes (LEDs) via multimode fiber. The design incorporated a custom imaging detector and a Micro-Electrical-Mechanical-Systems (MEMS) actuator for optical sectioning using structured …
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Deep learning for image processing in optical super-resolution microscopy
… live-cell imaging have specific requirements for illumination power and exposure time, thus necessitating a compromise with quality. In recent years, this fundamental limitation in optical microscopy has been shifted with the aid of deep learning methods. In this thesis, I propose methods that …
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Hochauflösende multispektrale Fluoreszenzmikroskopie mit lateralen stehenden Wellen
… objects were made. The new microscope offering structured illumination is especially suitable for biological applications since horizontally orientated slides can be observed from below. For multispectral measurements four different excitation wavelengths are available.
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Imaging and controlling atoms and semiconductor spins with advanced optical microscopy
… control [2], and (iii) high-throughput coherent structured illumination for controlling ultracold neutral atom arrays [3]. Optical microscopy has allowed revolutionary applications from life sciences to semiconductor industries. The field of microscopy is now undergoing another revolution as it …
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Methods for optical microscopy beyond conventional limits
… while maintaining the benefits of low intensity illumination and conventional sample mounting. An investigation into the possibility of further doubling the axial resolution of three-dimensional structured illumination microscopy (SIM) via a simple modification to the illumination system, and no …
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High Resolution Phase Imaging using Transport of Intensity Equation
… imaging technique by applying the principles of Structured Illumination Microscopy(SIM) to Transport of Intensity phase retrieval. The modulation from the illumination shifts the high frequency components of the phase object into the system pass-band. This enables phase imaging with resolutions …
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Circular Bessel field statistics and the pursuit of far-subwavelength resolution
… An imaging method based on object motion with structured illumination and far-field measurement data that results in far-subwavelength image information is proposed. Simulations show that this approach, with generous detector noise, will lead to the ability to distinguish image features on the …
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Optimal wavefronts and subwavelength structures : computer-aided design for optics and acoustics
… computational inverse design methods for structured illumination in holographic optical tweezers. Wave optimization is highly nonconvex by nature, and possesses many local optima due to interference and resonance. By combining a compact Bessel basis and a fast boundary element method, we …
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High resolution imaging and lithography using interference of light and surface plasmon waves
… reflection fluorescence (SW-TIRF), one of structured illumination techniques, with surface plasmon resonance (SPR). The SW-TIRF approach decreases the excitation wavelength by interfering two coherent light rays on the substrate and producing an evanescent standing wave field between the …
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Understanding the Organization, Location, and Interactions of Actin-based Structures During Drosophila Dorsal Closure
… components using total internal reflection structured illumination microscopy (TIRF-SIM). In concert with lattice light-sheet images, I show that when amnioserosa cells are relaxed, actin and myosin form a loose, domed meshwork that protrudes apically from the cellular junctions to which …
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