유전자 적중법을 이용한 조건적 LDHB 유전자 삭제 마우스 제작

In general, differentiated cells produce most of the ATP to growth from glucose through metabolism. Normal cells produce 2 ATP by glycolysis in the cytoplasm and convert to 2 pyruvates. Next, this pyruvate produces about 36 ATP by oxidative phosphorylation in the mitochondria. On the other hand, many metastatic cancer cells have altered glycolytic(glucose) metabolism. The lactate released by this altered glycolytic metabolism exported out of cancer cells. Because the tumor environment is highly hypoxic so that cancer cells produce of ATP by glycolysis. Cancer cells uptake glucose much more than normal cells. Because, they use inefficient glycolysis for producing ATP. In cancer cell, expression of gene associated with glycolysis and enzyme about lactate production are increased for activating glycolysis. Recently, Many report presented about that discovered altered glucose metabolism in cancer cells is not passive alteration by change in environment, but important role in normal cells. Lactate dehydrogenase(LDH) is a major enzyme involved in conversion of lactate in glycolysis. There are five LDH isozymes as a result of the five differenct combination that are produced by two subunit(A and B gene). Also, they have different enzymatic activity. The LDH-1 that consist of 4 LDHA complex is most active in LDH activity. But LDH activity is inverse amounts of LDHB. So we want make the condition of increased LDHA activity without LDHB in the cell. In this study, we designed a targeting vector about LDHB gene used by LDHB genome(mouse). And then, this targeting vector insert into the ES cell. Then targeted ES cell inject into blastocyst. As a result, we have got the chimera, LDHB targeted mouse. And now, we have gained the LDHB knockout mouse that generated by mating LDHB targeted mouse and Cre-expressed mouse. We expect that LDHB knockout mouse is used to animal model of research for cancer research and mitochondrial function that involved in cancer cell metabolism.

Author dc:creator

• 김, 소현

Contributors dc:contributor

• 박, 찬배
• 대학원 의생명과학과
• 200924336

• 유전자 적중법
• LDHB
• 마우스

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011684
000000011684

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/4383