C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication

Hepatitis B Virus (HBV) DNA polymerase (P) protein consisting of terminal protein (TP), spacer, reverse transcriptase (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated yet. In the present study, several chimeras of P protein by substituting Duck hepatitis B virus (DHBV) sequences were constructed. Accordingly, we tested a series of P protein chimeras in which several substitution mutants were disigned to contain various amino acids of DHBV P protein. It is found that amino acid residues from 800 to 826 (800SRPLLRLPFQPTTGRTSLYAVSPSVPS826) in C -terminus of the RNase H domain are required to complete HBV replication. HBV P protein mutants in which single amino acid residue was substituted were examined for the rescue of HBV replication. Among these mutants tested, L806T mutant P protein have a defect in pgRNA encapsidation and viral DNA synthesis, demonstrating that leucine at position 806 is critical for HBV replication.

Author dc:creator

• 김, 태영

Contributors dc:contributor

• 김, 경민
• 대학원 의생명과학과
• 200924464

• B형 간염 바이러스
• DNA 중합효소
• RNase H Domain
• C-말단
• C-Terminus
• Hepatitis B Virus
• HBV replication

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011459
000000011459

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/4358