Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"encapsidation"”.
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Transcription and encapsidation in parvoviruses LuIII and bovine parvovirus
… that identical ends are not required for the encapsidation of both DNA strands with equal frequency, as suggested for parvoviruses B19 and AAV. An infectious genomic clone of LuIII was constructed and sequenced. The LuIII genome is 5135 bases and it shares over 80% sequence identity with the …
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The encapsidation protein from Lactococcus lactis asccφ28 as a novel system for the study of molecular motors
… process. This process is thus driven by encapsidation proteins within large macromolecular motor complexes with energy derived from the hydrolysis of ATP. Indeed, Bacillus subtilis bacteriophage φ29 has served for many years as a model system for the study of phage motor complexes for its …
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Construction and evaluation of infectious cDNA clones and analysis of packaging determinants of Soybean dwarf virus
… in the 3’ UTR were involved in isolate-specific encapsidation of SbDV SsgRNA. Preferential trans-encapsidation of non-replicating mutant gRNAs and large subgenomic RNA (LsgRNA) by coat protein provided by replicating wild-type virus suggested that encapsidation of SbDV gRNA is replication …
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… that mutation of the C-box alone can enhance RNA encapsidation and mutation of the G-box can alter levels of Gag protein isoforms. These studies suggest coordinated regulation of RNA translation, dimerization, and encapsidation during HIV-2 replication. This research provides new insight into HIV …
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Characterization of 5 UTR splicing and the CGI in HIV-2 RNA
… that mutation of the C-box alone can enhance RNA encapsidation and mutation of the G-box can alter levels of Gag protein isoforms. These studies suggest coordinated regulation of RNA translation, dimerization, and encapsidation during HIV-2 replication. This research provides new insight into HIV …
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Functional Role of Hepatitis B Virus Core Protein in Viral Replication
… protein, supported HBV pregenomic RNA (pgRNA) encapsidation and DNA synthesis: 40% amino acid sequence identity or 45% homology in the nucleic-acid binding domain of HBV core protein was sufficient for pgRNA encapsidation and DNA synthesis, although we predominantly detected spliced DNA. A …
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The characterisation of aspects related to the single stranded RNA binding ability of African horsesickness virus nonstructural protein NS2
… the ten different virus genome segments during encapsidation, are proposed functions of NS2. This study investigates the binding ability of NS2 to ssRNA by looking at the protein structure, as well as its affinity for virus specific mRNAs. NS2 has an α-helix rich C-terminal region and α-helixes …
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Analysis, Optimization and Application of AAV Capsid Assembly using Escherichia coli
… delivery was also studied and strategies for DNA encapsidation were tested. Among 13 known human and nonhuman primate AAV serotypes, AAV serotype 2 (AAV2) is the most studied and AAV serotype 5 (AAV5) is the most genetically divergent serotype. To analyze AAV capsid assembly, AAV2 and AAV5 VP3 …
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Critical C-termical Amino Acids of Hepatitis B Virus Core Proteins for HBV Replication
… 구성되어있는 22kDa의 단백질이며, HBV core protein은 pgRNA 의 encapsidation 및 DNA 합성에 관여하는 것으로 알려져 있다. HBV core protein은 기능적으로 두 부위로 나뉜다. core particle 조립에 관여하는 core protein 의 N-말단부위와 핵산과 결합하는 부위인 C-말단부위로 나뉜다. Core protein의 N-말단부위 (amino acid 1-149)만으로도 core particle을 이루지만 pgRNA 는 encapsidation 을 하지 못한다. 이전 …
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SCREENING FOR POTENTIAL ANTIBACTERIAL AGENTS AND THE APPLICATION OF ISOTOPIC LABELS FOR THE CHARACTERIZATION OF THEIR EFFECTS ON THE DYNAMICS OF THE BACTERIAL 16S RIBOSOMAL RNA
… the HIV-2 promoter and human hepatitis B virus encapsidation signal epsilon element.
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C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication
… L806T mutant P protein have a defect in pgRNA encapsidation and viral DNA synthesis, demonstrating that leucine at position 806 is critical for HBV replication.
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Mechanisms of retroviral replication
Specific encapsidation of viral RNA is an essential step of retroviral replication cycle. Extensive biochemical and genetic analyses suggest that majority of retroviruses employ similar mechanisms for specific RNA packaging. Better understanding of mechanisms of this process may help to develop new …
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Structure of the GP16 ATPase from the φ29 dsDNA packaging motor
… as poxviruses, herpesviruses and adenoviruses. Encapsidation of bacteriophage φ29 DNA is driven by a phage-encoded molecular motor. This motor is powered by an oligomeric ATPase, gp16, or gene product 16, that converts energy obtained from ATP hydrolysis into translocation of dsDNA. In this …
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Optimization and characterisation of plant produced Human Papillomavirus pseudovirions in Nicotiana benthamiana
… 8Kb of pseudogenome DNA without the need of an encapsidation signal. HPV PsVs are used to test neutralising antibodies elicited by vaccines, for studying the virus life cycle, and potentially for delivery of therapeutic DNA vaccines. HPV PsVs are typically produced in mammalian cells; however, …
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RNA transmission and expression from inert HIV candidate vaccine virus-like-particles
… humoral immune responses in animal models. The encapsidation of large concentrations of random cellular RNA species is known to accompany the assembly of HIV virus particles. This RNA plays a crucial role by serving as a molecular scaffold for the assembly of Gag structural proteins into …
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Towards the development of plant-made PsVs as potential delivery vehicles for therapeutic HPV vaccines
… amplification (RCA) analysis showed successfully encapsidation of the E7SH-based pseudogenomes within the plant-made PsVs. However, TEM showed that PsV yields were low and no E7 expression was observed following infection of HEK293TT cells with the plant-made PsVs. These results indicated that …
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The role of the herpes simplex virus type 1 UL33 protein in DNA packaging
… and is required only during the latter stages of encapsidation. All sixteen UL33 mutants were again able to interact with both partners in immunofluorescence assays. Of the remaining HSV-1 proteins necessary for genome encapsidation, neither UL17 nor UL32 interacted with UL33. Immunofluorescence …
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