{"id":{"repo_id":"ajou","oai_identifier":"oai:repository.ajou.ac.kr:201003/4358"},"canonical_url":"https://search.dev.ndltd.org/etd/ajou/oai:repository.ajou.ac.kr:201003/4358","repository":{"repo_id":"ajou","name":"Ajou University","base_url":"http://repository.ajou.ac.kr/oai/request"},"display":{"title":"C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication","abstract":"Hepatitis B Virus (HBV) DNA polymerase (P) protein consisting of terminal protein (TP), spacer, reverse transcriptase (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated yet. In the present study, several chimeras of P protein by substituting Duck hepatitis B virus (DHBV) sequences were constructed. Accordingly, we tested a series of P protein chimeras in which several substitution mutants were disigned to contain various amino acids of DHBV P protein. It is found that amino acid residues from 800 to 826 (800SRPLLRLPFQPTTGRTSLYAVSPSVPS826) in C -terminus of the RNase H domain are required to complete HBV replication. HBV P protein mutants in which single amino acid residue was substituted were examined for the rescue of HBV replication. Among these mutants tested, L806T mutant P protein have a defect in pgRNA encapsidation and viral DNA synthesis, demonstrating that leucine at position 806 is critical for HBV replication.","abstract_html":"Hepatitis B Virus (HBV) DNA polymerase (P) protein consisting of terminal protein (TP), spacer, reverse transcriptase (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated yet. In the present study, several chimeras of P protein by substituting Duck hepatitis B virus (DHBV) sequences were constructed. Accordingly, we tested a series of P protein chimeras in which several substitution mutants were disigned to contain various amino acids of DHBV P protein. It is found that amino acid residues from 800 to 826 (800SRPLLRLPFQPTTGRTSLYAVSPSVPS826) in C -terminus of the RNase H domain are required to complete HBV replication. HBV P protein mutants in which single amino acid residue was substituted were examined for the rescue of HBV replication. Among these mutants tested, L806T mutant P protein have a defect in pgRNA encapsidation and viral DNA synthesis, demonstrating that leucine at position 806 is critical for HBV replication.","abstract_has_math":false,"creators":["김, 태영"],"institution":null,"degree_name":null,"degree_level":null,"degree_discipline":null,"degree_department":null,"school":null,"contributors":["김, 경민","대학원 의생명과학과","200924464"],"advisors":[],"committee_chairs":[],"committee_members":[],"year":2011,"date_issued":"2011-11-07T02:17:00Z","date_published":"2011-11-07T02:17:00Z","updated_at":"2026-07-24T00:51:36Z","subjects":["B형 간염 바이러스","DNA 중합효소","RNase H Domain","C-말단","C-Terminus","Hepatitis B Virus","HBV replication"],"languages":["en"],"rights":[],"rights_urls":[],"identifier_entries":[{"key":"dc:identifier","label":"Identifier","values":["http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011459","000000011459"],"render_values":[{"text":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011459","href":"http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011459","code":true},{"text":"000000011459","href":null,"code":true}]}]},"links":{"outbound_url":"http://repository.ajou.ac.kr/handle/201003/4358","outbound_label":"Repository record","outbound_source":"dc:identifier"},"metadata_groups":[{"id":"people","label":"People","entries":[{"key":"dc:contributor","label":"Contributor","values":["김, 경민","대학원 의생명과학과","200924464","김, 태영"]},{"key":"dc:creator","label":"Author","values":["김, 태영"]}]},{"id":"academic_context","label":"Academic Context","entries":[{"key":"dc:date","label":"Dc Date","values":["2011-11-07T02:17:00Z","2011"]},{"key":"dc:type","label":"Dc Type","values":["Thesis","Theses"]}]},{"id":"subjects_keywords","label":"Subjects and Keywords","entries":[{"key":"dc:subject","label":"Dc Subject","values":["B형 간염 바이러스","DNA 중합효소","RNase H Domain","C-말단","C-Terminus","Hepatitis B Virus","HBV replication"]}]},{"id":"language_rights","label":"Language and Rights","entries":[{"key":"dc:language","label":"Dc Language","values":["en"]}]},{"id":"identifiers","label":"Identifiers","entries":[{"key":"dc:identifier","label":"Identifier","values":["http://repository.ajou.ac.kr/handle/201003/4358","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011459","000000011459"]}]},{"id":"additional","label":"Additional Metadata","entries":[{"key":"dc:description","label":"Description","values":["Hepatitis B Virus (HBV) DNA polymerase (P) protein consisting of terminal protein (TP), spacer, reverse transcriptase (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated yet. In the present study, several chimeras of P protein by substituting Duck hepatitis B virus (DHBV) sequences were constructed. Accordingly, we tested a series of P protein chimeras in which several substitution mutants were disigned to contain various amino acids of DHBV P protein. It is found that amino acid residues from 800 to 826 (800SRPLLRLPFQPTTGRTSLYAVSPSVPS826) in C -terminus of the RNase H domain are required to complete HBV replication. HBV P protein mutants in which single amino acid residue was substituted were examined for the rescue of HBV replication. Among these mutants tested, L806T mutant P protein have a defect in pgRNA encapsidation and viral DNA synthesis, demonstrating that leucine at position 806 is critical for HBV replication.","Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 5 A. HBV plasmid DNA construction 5 B. Cell culture and transfection 8 C. Isolation of core particles 9 D. RNase protection assay (RPA) 9 E. Core particle Western blotting 10 F. Southern blotting 11 G. SDS-PAGE and Western blotting 11 Ⅲ. RESULT 12 A. HBV P constructs containing DHBV P residues in RNase H domain 12 B. Amino acid residues from 800 to 826 in C-terminus of the RNase H are critical for pgRNA encapsidaion and DNA synthesis. 14 C. The small motif substituted HBV RNase H domain mutants at C-terminus have ability to support HBV DNA synthesis. 21 D. The small motif substituted HBV RNase H domain mutants at C-terminus have ability to support HBV pgRNA encapsidation. 23 E. A leucine residue at position 806 in HBV P protein is important for viral genome replication. 26 F. A leucine residue at position 806 in HBV P protein is important for pgRNA encapsidation. 28 Ⅳ. DISCUSSION 29 Ⅴ. CONCLUSION 31 REFERENCES 32 국문요약 38","Master"]},{"key":"dc:title","label":"Title","values":["C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication","B형 간염 바이러스 DNA중합효소 RNase H domain 의 C-말단이 바이러스 증식에 미치는 영향"]}]}],"canonical_facts":{"dc:contributor":["김, 경민","대학원 의생명과학과","200924464","김, 태영"],"dc:creator":["김, 태영"],"dc:date":["2011-11-07T02:17:00Z","2011"],"dc:description":["Hepatitis B Virus (HBV) DNA polymerase (P) protein consisting of terminal protein (TP), spacer, reverse transcriptase (RT), and RNase H, plays critical roles in viral assembly and replication. RNase H domain is required for HBV DNA replication, however critical motif or amino acid residues in the RNase H domain for the HBV replication has not been extrensively demonstrated yet. In the present study, several chimeras of P protein by substituting Duck hepatitis B virus (DHBV) sequences were constructed. Accordingly, we tested a series of P protein chimeras in which several substitution mutants were disigned to contain various amino acids of DHBV P protein. It is found that amino acid residues from 800 to 826 (800SRPLLRLPFQPTTGRTSLYAVSPSVPS826) in C -terminus of the RNase H domain are required to complete HBV replication. HBV P protein mutants in which single amino acid residue was substituted were examined for the rescue of HBV replication. Among these mutants tested, L806T mutant P protein have a defect in pgRNA encapsidation and viral DNA synthesis, demonstrating that leucine at position 806 is critical for HBV replication.","Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 5 A. HBV plasmid DNA construction 5 B. Cell culture and transfection 8 C. Isolation of core particles 9 D. RNase protection assay (RPA) 9 E. Core particle Western blotting 10 F. Southern blotting 11 G. SDS-PAGE and Western blotting 11 Ⅲ. RESULT 12 A. HBV P constructs containing DHBV P residues in RNase H domain 12 B. Amino acid residues from 800 to 826 in C-terminus of the RNase H are critical for pgRNA encapsidaion and DNA synthesis. 14 C. The small motif substituted HBV RNase H domain mutants at C-terminus have ability to support HBV DNA synthesis. 21 D. The small motif substituted HBV RNase H domain mutants at C-terminus have ability to support HBV pgRNA encapsidation. 23 E. A leucine residue at position 806 in HBV P protein is important for viral genome replication. 26 F. A leucine residue at position 806 in HBV P protein is important for pgRNA encapsidation. 28 Ⅳ. DISCUSSION 29 Ⅴ. CONCLUSION 31 REFERENCES 32 국문요약 38","Master"],"dc:identifier":["http://repository.ajou.ac.kr/handle/201003/4358","http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011459","000000011459"],"dc:language":["en"],"dc:subject":["B형 간염 바이러스","DNA 중합효소","RNase H Domain","C-말단","C-Terminus","Hepatitis B Virus","HBV replication"],"dc:title":["C-terminus of Hepatitis B virus RNase H Domain is Important for HBV replication","B형 간염 바이러스 DNA중합효소 RNase H domain 의 C-말단이 바이러스 증식에 미치는 영향"],"dc:type":["Thesis","Theses"]},"updated_at":"2026-07-24T00:51:36Z"}