Relationship between Hepatitis B Virus Core Particles and Intracellular Motility

PURPOSE: Hepatitis B virus (HBV) core particles recycle back to the nucleus. This recycling mechanism is thought to be important in maintaining covalently closed circular (cccDNA) pool in the nucleus. Transport of HBV core particles in host cytoplasm is important for HBV life cycle but the mechanism is not elucidated yet. In this study, the change of the cytoskeletal organization and organelle distribution was observed in HBV replicating stable cell lines. Also, how HBV core particles were transported in cytoplasm was elucidated. MATERIALS & METHODS: HBV replicating stable cell lines were established in hepatoma cell lines, HuH7 and HepG2 cells. Using Northern blot and Southern blot analysis, HBV transcription and replication were detected in HBV replicating stable cell lines. Distribution of intracellular organelles was elucidated by immunfluorescence staining. Mitochondria were stained by MitoTracker. And microtubules (MTs) was visualized using anti-tubulin antibody. RESULTS: The mitochondrial aggregation towards the nucleus was observed in HBV replicating stable cells, but not in HuH7 and HepG2 cells. When HBV replicating stable cells were treated with a MT disrupting drug, nocodazole, mitochondria dispersed throughout the cytoplasm, which indicates that the microtubule network is indispensable in mitochondrial aggregation. Interestingly, removal of nocodazole caused a rapid reaggregation of mitochondria in 30 min, suggesting that the minus end-directed motor protein, dynein, might be actively involved. ER and peroxisome are aggregated to perinuclear region like mitochondria in HBV replicating stable cells. Also, HBV core particles and MTs were colocalized in HBV replicating cells, indicating that HBV core particles may be associated with MTs. CONCLUSION: These results suggest that HBV replication induce the rearrangement of MT network, and dynein, the MT- based minus end-directed motor protein, might be actively involved in this process. The incoming and recycling HBV core particles might be propelled along MTs by dynein.

Author dc:creator

• 김, 혜영

Contributors dc:contributor

• 김, 경민
• 대학원 의학과
• 105281

• Hepatitis B virus
• core particles
• mitochondrial aggregation
• microtubule
• B형 간염 바이러스 바이러스
• core 입자
• 미토콘드리아 응집
• 미세 소관

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000000085
000000000085

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/2289