Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 441 for “"microtubule"”.
-
Microtubule Dynamics in Neuronal Morphogenesis
Microtubules in neurons provide structural stability, and also provide a transport route for cellular cargo carried by motor proteins. Exactly how microtubules are organized and maintained in neurons is not clear, but microtubule organizing centers (MTOCs) in neurons are not required as in …
-
Insight into Microtubule Catastrophe and Growth
Pages ii-xi are misnumbered as pages i-x.
-
Molecular mechanisms of kinetochore microtubule attachment
… must remain attached to depolymerizing microtubules, which drive poleward chromosome movement. Microtubules are highly dynamic structures that undergo dramatic structural changes during depolymerization. The results presented in this thesis define essential functions of the …
-
Mechanistic Analysis of Microtubule Dynamics and Regulation
Microtubules are critical components in a cells cytoskeletal network, known to form the mitotic spindle which allows for chromosome segregation and cell division and organizing the cytoplasm of non-dividing cells. The quick reorganization of the cytoskeleton relies heavily on the underlying …
-
Examining Mechanisms Regulating Microtubule Assembly and Function
<p>Microtubules are cytoskeletal polymers assembled from α and β tubulin subunits that function in essentially all cellular activities. Microtubules can act as "tracks" for intracellular cargo transport, are required for cilia- and flagella-based motility, and establish cell morphology in …
-
Characterization of chartins, cytoplasmic microtubule associated proteins
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1987.
-
Functional analyses of mitotic microtubule-binding complexes
… complex, termed the kinetochore, to microtubules. Microtubules are dynamic polymers comprised of tubulin heterodimers. The successful execution of mitosis additionally depends on the organization of the microtubules into a bipolar array, termed the mitotic spindle. The …
-
Structure-Based Mechano-Chemical Modeling of Microtubule Nucleation
Microtubule (MT) nucleation is essential for organizing and regulating the cellular MT network. Nucleation begins from two types of templates: 1) Seed MTs – tubelike templates resembling normal MTs, and 2) γ-tubulin ring complexes (γTuRCs) – dynamic templates that must undergo closure to become …
-
Self-organization of Microtubule and Associated Protein Map65
… in vitro system containing cytoskeletal fiber microtubule and associated protein MAP65, I try to address self-organization through passive entropic force mechanisms. I use techniques like protein purification, light microscopy, and image analysis to quantify my results. First, I show how …
-
Modelling the formation of ordered acentrosomal microtubule arrays
Acentrosomal microtubules are not bound to a microtubule organising centre yet are still able to form ordered arrays. Two clear examples of this behaviour are the acentrosomal apico-basal (side wall) array in epithelial cells and the parallel organisation of plant cortical microtubules. This …
-
Microtubule Organisation in Normal and Colon Cancer Cells
… function," with" the" rearrangement" of" the"microtubule" cytoskeleton" on" differentiation" critical" for" the" establishment" of" polarity." "During" this" process,"microtubules" reorganise" to" form"a"stabilised"array"of"apicoNbasal"bundles"via"a"release"and"capture"mechanism."" …
-
Kinetochore-Microtubule interface: analyses of Ndc80-Dam1 Interactions
… chromosome segregation can only occur when microtubules capture kinetochores and align sister chromatids on the mitotic spindle. Initially kinetochores interact with the lateral surface of a microtubule (lateral attachment), which is subsequently converted to an end-on attachment when the …
-
Role of Microtubule Motor Proteins in Adenoviral Infections
… Interestingly, adenovirus relies on the microtubule (MT) network, MT-dependent motor proteins, and virus-stimulated cAMP-activated kinase (protein kinase A, PKA) activity to traverse the cytoplasm during the critical intracellular transport phase between endosomal escape and nuclear pore …
-
Bidirectional cargo transport by microtubule-based molecular motors
We have reconstituted a simple in vitro system using only mammalian dynein and mammalian kinesin attached to a single cargo. These cargoes undergo saltatory motion typically seen in vivo, indicating that the motors engage in a tug-of-war. When the complex hits a barrier, the cargo often reverses …
-
Exploiting AI to understand microtubule orientation in neurons
In neuronal axons, almost all microtubules are oriented with their growing end (+end) away from the cell body (they are oriented ‘+end out’). Molecular motor proteins rely on this orientation to efficiently move vital cargo to the distal regions of the axon. Despite 30 years of research, the …
-
KINESIN-MICROTUBULE INTERACTIONS DURING GLIDING ASSAYS UNDER MAGNETIC FORCE
Conventional kinesin is a motor protein capable of converting the chemical energy of ATP into mechanical work. In the cell, this is used to actively transport vesicles through the intracellular matrix. The relationship between the velocity of a single kinesin, as it works against an increasing …
-
Modelling and image processing of microtubule dynamics and organisation
Microtubules, dynamic protein polymers, form networks that are essential for intracellular organisation. Involved in many cellular processes that are vital in development and homeostasis, improper regulation of the microtubule network is implicated in various diseases. This work addresses the …
-
Molecular analysis of kinetochore-microtubule attachment in budding yeast
Kinetochores bind to microtubules and are responsible for chromosome segregation and the accurate transmission of genetic information during cell division. Kinetochores are DNA-protein complexes that assemble on centromeric DNA sequences. In budding yeast, the kinetochores consist of approximately …
-
Site-directed mutagenesis of the ncd microtubule motor protein
… of MC6-A623 (under the conditions tested) for microtubules was less than MC6. Most interestingly, under the conditions tested, MC6-A623 did not exhibit an increased ATPase rate in the presence of microtubules, a hallmark of the kinesin family of microtubule motor proteins. Analysis of the …
Page 1 of 23