Coronavirus Replication Inhibition by Herbal Extracts

Coronaviruses are plus-stranded RNA viruses that infect human and large variety of animals, including pig, cattle, dog, rat, rabbit, turkey, and bird. Human coronavirus has been known to cause common colds and perhaps gastroenteritis. Animal coronaviruses cause gastroenteritis, hepatitis, and a syndrome similar to multiple sclerosis of humans. The resent severe acute respiratory syndrome (SARS) outbreak has stirred out global, which turned out to be a novel zoonotic coronavirus. SARS is an infectious disease with a high potential for transmission by close contact. In search for the active viral compound to treat coronavirus infections including SARS coronavirus, I screened several herbal extracts from natural compounds affecting replication of mouse hepatitis virus (MHV), the prototype coronavirus, infected cells. The reduced virus production was analyzed by plaque assay. The replication inhibition of MHV by herbal extracts in terms of RNA replication was explored through the analysis of the intracellular viral RNAs. Plaque assay results showed that some of the herbal extracts inhibited coronavirus replication. Northern blot analyses showed that the intracellular viral RNA expressions were delayed by the herbal extracts. At present, several herbal extracts were identified that are effective for MHV reduction in cell culture system: Inula helenium (목향); Ledebouriella seseloides (방풍); Bupleururn lalcalum (시호); Artemisia sp (애엽); Artemisia japonica (청호); Myristica fragrans (초과); Amur cork tree (황백); Scutellaria baicalen (황금); Lonicera japonica (금은화); Forsythia Korea (연교); Reynoutria elliptic (호장근); Houttuynia cordata (어성초); Paeonia suffruticosa (목단피); Rheum undulatum (대황); Epimedium koreanum (음양곽).

Author dc:creator

• 신, 현수

Contributors dc:contributor

• 김, 경민
• 대학원 의학과
• 200524188

• Coronavirus
• 한약재
• 한약재 스크리닝
• 코로나바이러스
• 억제제

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002038
000000002038

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1435