The role of tubulin in ganglioside-induced ramifications in cultured rat brain microglia

"In addition to functional changes, activated microglia undergo morphological changes from a resting, ramified morphology to an activated, amoeboid shape. Gangliosides, despite their role as microglial activators, induce microglial ramifications. To identify the cytoskeletal components involved in microglial ramification, we stimulated cells with gangliosides in the absence or presence of cytoskeletal inhibitors and examined the resultant morphological changes. While LPS-induced ameboid shapes were blocked by all inhibitors, actin inhitibors and microtubule inhibitor, Ganglioside-induced ramifications were blocked by either paclitaxel or nocodazole, inhibitors of microtubule, but not by cytochalasin B or latrunculin B, which are inhibitors of actin polymerization. In contrast to these morphological changes, iNOS expression was suppressed by both inhibitors of actin filament and inhibitors of microtubule. These results indicate that ganglioside-induced ramification is more dependent on tubulin than actin and that both of these cytoskeletal components are involved in iNOS expression. "

Author dc:creator

• 박, 지영

Contributors dc:contributor

• 주, 일로
• 대학원 의학과
• 200324504

• tubulin
• actin
• gangliosides
• microglia
• ramification

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000001228
000000001228

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1402