Role of Extracellular Signal­-Regulated Kinase and Peroxisome Proliferator-­Activated Receptor gamma on Transforming Growth Factor-­β1-­Induced Human Endometrial Stromal Cell Decidualization

OBJECTIVE: To investigate the role of ERK and PPAR gamma on TGF-β1-induced human endometrial stromal cell decidualization in vitro. MATERIALS AND METHODS: Human endometrial tissues obtained by hysterectomy specimens from patients with conditions other than endometrial diseases such as leiomyoma. Endometrial stromal cells were cultured under the following conditions: DMEM/F-12 (10% FBS, 1 nM E2 and 100 nM P4). 5 ng/ml of TGF-β1, 50 nM of rosiglitazone (PPAR gamma agonist), and 20 μM of PD98059 (ERK inhibitor) were added according to experimental purposes. Trypan-blue and a hemocytometer were utilized to count the viable cell number. Enzyme-linked immunosorbent assay (ELISA) and Western blotting was employed to detect proteins. RESULTS: TGF-β1 inhibited proliferation of cultured human endometrial stromal cells and induced expression of prostaglandin E2 (PGE2) and prolactin. This effect was mediated by Smad and ERK activation. Addition of rosiglitazone, a PPAR gamma agonist, prevented TGF-β1 effect on endometrial cell proliferation. Furthermore, rosiglitazone inhibited TGF-β1 induced activation of ERK, consequently reducing PGE2 and prolactin production. CONCLUSION: TGF-β1-induced decidualization of endometrial stromal cells through Smad and ERK phosphorylation. PPAR gamma acts as a negative regulator of human endometrial cell decidualization in vitro.

Author dc:creator

• 장, 혜진

Contributors dc:contributor

• 황, 경주
• 대학원 의학과
• 103765

• Decidualization
• TGF-β1
• PPAR gamma
• 자궁내막
• 탈락막화

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000002027
000000002027

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/1295