Chronic Ocular Hypertensive Model using Microbead Injection in Rats: Comparison of Polyurethane, Polymethylmethacrylate, and Polystyrene

Purpose: To establish and assess an ocular hypertensive rat model using intracameral injection with various microbeads of different sizes and materials. Methods: Chronic elevation of intraocular pressure (IOP) was induced by injection of various microbeads into the anterior chamber of Sprague-Dawley rat eyes. We compared the IOPs induced by the injection of different microbeads [7- and 17-µm polyurethane (PU), 7- and 15-µm polymethylmethacrylate (PMMA), and 15-µm polystyrene (PS)] and selected the appropriate microbeads for a chronic ocular hypertensive model in terms of IOP elevation and adverse events. IOP changes were observed for 4 weeks after microbead injections. Axonal degeneration was assessed with transmission electron microscopic photographs and RGC loss was assessed with retrograde labeling. Results: Sixty-nine rats were included. Three days after a single injection of microbeads, IOPs were increased by 24.0% by 7-µm PU microbeads, 101.8% by 17-µm PU microbeads, 56.6% by 7-µm PMMA microbeads, 22.0% by 15-µm PMMA microbeads, and 34.7% by 15- µm PS microbeads. 17-µm PU microbeads produced constant IOP elevation with good reproducibility (standard deviation of < 6.6 mmHg). Sustained IOP elevation by two injections of 17-µm PU microbeads resulted in a 42% axon loss and 36.5% RGC loss (p<0.05, Mann-Whitney U test). Conclusions: PU microbead injections offer an applicable and versatile model for a chronic ocular hypertensive model in rats. Among several biomaterials, PU microbeads produced a more stable IOP elevation without adverse events.

Author dc:creator

• 노, 승수

Contributors dc:contributor

• 안, 재홍
• 대학원 의학과
• 200524171

• Chronic ocular hypertension
• Microbead
• Axon
• Retinal ganglion cell
• Intraocular pressure
• Polyurethane
• 만성 고안압증
• 미세구슬
• 축삭돌기
• 망막신경절세포
• 안압
• 폴리우레탄

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000020213
000000020213

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/11892