Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv

A subset of anti-DNA antibodies (Ab) can internalize in a variety of cells. An anti-nucleic acid antibody, murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv internalization remains unknown. Here, we studied for cell surface receptor of 3D8 scFv for cellular internalization. 3D8 scFv bound to both cell surface heparan sulfate proteoglycans (HSPGs) and chondroitin sulfate proteoglycans (CSPGs) that are highly negatively charged molecules known as endocytic receptors for a variety of ligands. Treatment of soluble HS or CS reduced cell surface binding of 3D8 scFv to intact HSPGs. Intracellular co-localizations of three molecules were observed between 3D8 scFv-HSPGs-caveolin 1, and 3D8 scFv-CSPGs-caveolin 1 in HeLa cells. Furthermore, internalization of 3D8 scFv was significantly reduced in HS/CS-deficient CHO cells (pgsA-745), compared to in wild-type CHO cells. These data provide the evidence for both HSPGs and CSPGs as internalizing receptor of 3D8 scFv.

Author dc:creator

• 김, 혜진

Contributors dc:contributor

• 권, 명희
• 대학원 의생명과학과
• 201224126

• Anti-nucleic acid antibody
• 3D8 scFv
• Internalizing receptor
• HSPGs
• CSPGs
• 항-핵산항체
• 세포내 이입 수용체
• 헤파란황산 단백당
• 콘드로이틴황산 단백당

Language dc:language

en

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017735
000000017735

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/10871