Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting

3D8 single chain variable fragment (scFv) derived from a MRL-lpr/lpr mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against replication of RNA-genomed viruses and this anti-viral activity should be due to the cellular internalizaing and DNA/RNA- hydrolyzing activities of 3D8 scFv. To extend the possible applications of anti-viral activity of 3D8 scFv, we have developed a chickenized antibody of 3D8 scFv by CDRs (complementarity-derermining regions) grafting. A chickenized 3D8 scFv variant (CK) was generated by transferring six CDRs of 3D8 scFv between FRs (framework regions) of a chicken antibody which reveals high solubility and expression level in bacterial culture. CK6 of chickenized 3D8 scFv variant (CK1~7) retained the DNA-binding, DNA-hydrolysis, and cellular internalizing activities similar to that of the original mouse 3D8 scFv (m3D8). Our results demonstrate that chckenized 3D8 scFv can be generated by grafting CDRs.

Author dc:creator

• 노, 주호

Contributors dc:contributor

• 권, 명희
• 대학원 의생명과학과
• 201224127

• 마우스 3D8 scFv
• 항-핵산 항체
• CDR-이식
• 닭화 (chickenized)
• 3D8 scFv
• anti nucleic acid antibody
• DNA/RNA hydrolyzing activity
• chickenization
• CDR-grafting
• Immunogenicity

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000016301
000000016301

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/10851