Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"3D8"”.
-
Effect of disruption of 3D8 complementarity-determining regions on properties of 3D8 antibody
… CDR-disruption on the function of an antibody, 3D8 scFv (single chain variable fragment; variable region of heavy chain connected to variable region of light chain by (G4S1)3 linker) which has DNA-binding/-hydrolyzing, heparin-binding and cell-penetrating activities. We generated single …
-
Antiviral activity of 3D8 single chain Fv against RNA virus infection
… fields to target viral antigens effectively. 3D8 is an anti-DNA catalytic antibody that binds to DNA and RNA then degrades them without sequence specificity as well known as Abzymes. Previously it was reported that 3D8 single chain variable fragment (scFv) has cell-penetrating activity by …
-
Suppressive effect of a nucleic acid-hydrolyzing recombinant antibody (3D8) on viral multiplication
3D8 single chain variable fragment (scFv) is a recombinant anti-DNA antibody from an autoimmune-prone MRL-lpr/lpr mouse with lupus diseases. 3D8 scFv can hydrolyze ss-DNA, ds-DNA, ss-RNA, and ds-RNA without sequence specificity. On the base of 3D8 scFv property, we carried out quantitative and …
-
Generation of chickenized 3D8 scFv antibody from mouse monoclonal anti-DNA antibody by CDRs-grafting
3D8 single chain variable fragment (scFv) derived from a MRL-lpr/lpr mouse is a catalyric anti nucleic acid antibody that has ability of cellular internalization and DNA/RNA-hydrolyzing activity. It has been reported that cells treated with 3D8 scFv protein showed the down-regulation against …
-
Identification of the receptor for cellular internalization of an anti-nucleic acid antibody, 3D8 scFv
… of cells. An anti-nucleic acid antibody, murine 3D8 single chain variable fragment (scFv) Ab, has been known to be an internalizing antibody that enters the cells by caveolae/lipid-raft-mediated endocytosis. However, cell surface receptor for 3D8 scFv internalization remains unknown. Here, we …
-
Characterization of anti-DNA antibody with Tat peptide sequence in CDR
"Tat 펩티드가 삽입된 항-DNA 항체 구조의 생화학적 특성 항-DNA 항체인 3D8 단일 가닥 가변부위 (single chain variable fragment: scFv)는 핵산결합능 및 가수분해활성이 있으며, 3D8 VL 단독부위만으로도 핵산 결합능 및 가수분해능을 발휘한다. 또한 3D8 scFv는 세포막 침투능이 있어서, 세포막을 투과한 후, 세포질에 위치하게 되고, 결국은 세포사멸을 유도한다고 알려져 있다. 본 연구에서는 3D8 scFv의 핵산가수분해 활성은 그대로 유지하면서, 세포막 투과 후 핵으로 이동할 수 있는 …
-
Anti-Nucleic Acid Antibody-Mediated Cross Presentation of An Antigen and Its CD8+ T Cell Activation
… (scFv) of a nucleic acid-hydrolyzing antibody, 3D8. As the CD8-T cell epitopes, chicken ovalbumin amino acid 257-264 (OVA257-264-SIINFEKL) and 250-264 (OVA250-264-SGLEQLESIINFEKL) were used for 3D8 scFv fusion partners. Purified 3D8 scFv-OVA fusion proteins with ~95% purity retained DNA-binding, …
-
동물 세포의 세포질에서 발현된 IgG 의 중쇄와 경쇄간의 조합 여부 및 항원결합 활성 분석
… 및 항원 결합능력 유지 여부가 자세하게 연구된 것이 없다. 따라서 본 연구에서는 키메릭 3D8 IgG1 (chimeric 3D8 IgG1, ch 3D8 IgG1) 발현벡터를 세포질에 발현시켜 중쇄와 경쇄간의 결합 및 항원 결합능력을 조사하였다. 세포질에서 발현되도록 선도 서열 (leader sequence)을 제거하여 중쇄와 경쇄를 동시에 발현하는 벡터를 제조하였다. ch 3D8 IgG1의 기본구조 외에도 중쇄의 경첩 부위 (hinge region) 및 중쇄-경쇄 사이의 cys 잔기를 세린 (Serine, Ser) 잔기로 치환한 …
-
Functional stability of a nucleic acid-hydrolyzing single chain antibody in various biochemical and biophysical environments
3D8 single chain variable fragment (scFv) antibody has cell-penetrating activity, nucleic acid-binding activity, nucleic acid-hydrolyzing activity, and antiviral activity. As a first step toward development of antiviral reagent using 3D8 scFv, functional stability based on DNA-binding and …
-
A Pseudoknot Improves Selection Efficiency in Ribosome Display
… I used the previously-isolated anti-DNA scFv, 3D8, as a target protein. During in vitro translation in rabbit reticulocyte lysate, I observed that incorporation of the pseudoknot into the mRNA resulted in production of a translational intermediate that corresponded to the expected size for …