TIS21 inhibits cell migration by downregulating expression of matrix metalloproteinases

Tetradecanoyl phorbol acetate-inducible sequence 21 (TIS21) is the mouse homolog of rat pheochromocytoma cell 3 (PC3) and human B-cell translocation gene 2 (BTG2). TIS21 regulates cell cycle progression and proneural genes expression by acting as a transcription coregulator that enhances or inhibits the activity of transcription factors. And In human tissue, BTG2 has a critical role in cancer cell migration and invasion. The matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that are capable of degrading all kinds of extracellular matrix proteins, but also can process a number of bioactive molecules, and to perform key functions in tumor progression, and in other diseases. In this study, we report that TIS21 can decrease the expression at both mRNA and protein levels of MMPs in human breast cancer (MDA-MB-231) cells and human dermal fibroblasts (HDF) cells, as confirmed by zymography, RT-PCR. Moreover we demonstrate that TIS21 decreases transcription factor (c-Fos, PEA3) of MMPs. We are going to examine the regulatory mechanism of TIS21-mediated MMPs proteoytic activity.

Author dc:creator

• 최, 명호

Contributors dc:contributor

• 박, 태준
• 대학원 의생명과학과
• 200924344

• TIS21/BTG2/PC3
• MMPs 의 mRNA 발현과 단백질 활성도 감소
• MDA-MB-231
• HDF 세포의 이동성 감소
• downregulation mRNA expression and proteolytic activity of MMPs
• downregulation migration of MDA-MB-231

Language dc:language

ko

Identifier

http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000016270
000000016270

OAI identifier oai:identifier

oai:repository.ajou.ac.kr:201003/10849