Engineering and use of plant viral expression vectors

Different plant virus-based expression systems were developed and compared using the colorectal cancer antibody CO17-1A and the corresponding antigen GA733-2 as model proteins. Tobacco mosaic virus (TMV) and alfalfa mosaic virus (AlMV) were used to construct the vector backbones of four systems to express multipartite proteins, single proteins or domains thereof. A fully infectious TMV-based vector was compared with a TMV-AlMV helper virus system consisting of a mixture of an infectious and a replication deficient virus. In addition, an AlMV RNA3 complementation vector was developed consisting of two defective RNA3s that complement each other in movement functions. The fourth vector for recombinant protein domain presentation on the assembled virus particle was also based on AlMV. Antibody-binding domains of antigen GA733-2 were introduced into the coat protein of AlMV. Recombinant protein expression was demonstrated with all systems tested. Differences of vector efficiency were assayed in terms of genetic vector stability, recombinant protein yields and the ability to systemically infect the plants. Further detailed studies of plant virus vector systems are being undertaken to optimize stability and efficiency of newly developed plant expression systems.

Grantor dc:publisher

Publikationsserver der RWTH Aachen University

Year dc:date

2000

Author dc:creator

• Verch, Thorsten

Contributors dc:contributor

• Kreuzaler, Fritz

• info:eu-repo/classification/ddc/570
• Biowissenschaften, Biologie
• Pflanzenviren
• Expressionsvektor

Statement dc:rights

• info:eu-repo/semantics/openAccess

Language dc:language

eng

Identifier

https://publications.rwth-aachen.de/search?p=id:%22RWTH-CONV-118359%22

OAI record oai:identifier

https://publications.rwth-aachen.de/oai2d?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Apublications.rwth-aachen.de%3A56241