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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 51 for “"expression vectors"”.
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Construction and analysis of novel controllable expression vectors for Bacillus subtilis
… acquired. But so far, efficient and inexpensive expression vectors for B. subtilis are still missing. To fill this gap, a glycine-inducible expression system and a lysine-autoinducible one were explored and IPTG-inducible expression plasmids that allow overexpression and purification of proteins …
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Enhancement of plant expression vectors using replication and silencing suppressor elements
… elements were used with the aim of increasing expression and yield of enhanced green fluorescent protein (EGFP) and the Zika PrME polyprotein in Nicotiana benthamiana. A comparison of four viral silencing suppressor proteins was performed: these were tomato spotted wilt virus non-structural …
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Characterization of two, desiccation linked, Group 1 LEA proteins from the resurrection plant Xerophyta humilis
… plants and other anhydrobiotic organisms show expression of Late Embryogenesis Abundant (LEA) proteins associated with desiccation tolerance. However, the precise role of these proteins has not been described. This study was undertaken to investigate expression, structure and function of …
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Construction of plasmid-based expression and secretion vectors and study of the immobilization of proteins on the surface of Bacillus subtilis cells
Plasmids are useful tools to study gene expression and their function. However, most of the available plasmids for Bacillus subtilis suffer from structural instability because of their rolling-circle replication mechanism. In this work, stable plasmids have been constructed allowing expression of …
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Development of microalgae as a biopharming platform
… microalgae transient production platform for the expression of recombinant protein. First it had to be established whether the available plant expression vectors could be used for the transfer of genetic material into packed microalgae. The method showed successful transfer of the neomycin …
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Single-Stranded-Dna Sp6 and T7 Bacteriophage Promoter Plasmids for Engineering Mutant Rnas and Proteins: Extensions and Deletions at the Amino Terminus Which Alter the Activity of Preproparathyroid Hormone Signal Peptide
… mutagenesis, enzymatic sequence analysis, and expression of mutant proteins, all from a single vector. Chimeric phage-plasmid expression vectors, or phagemids, containing the intergenic region of the single stranded DNA bacteriophage f1 and either a bacteriophage SP6 or T7 promoter have been …
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MOLECULAR TROJAN HORSES IN THE TREATMENT OF METACHROMATIC LEUKODYSTROPHY
… in eukaryotic cells transduced with lentiviral expression vectors and have been assessed for in vitro enzymatic activity and kinetic properties. Although preliminary studies show that these constructs did not cross an in vitro BBB model under these experimental conditions, there is evidence to …
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Generation and Characterization of dszB Mutants from Nocardia asteroides A3H1
… into the pBAD TOPO® and pBAD TOPO®/ThioFusion expression vectors. Cloning efficiency of the wildtype gene into the pBAD TOPO® vector was 86%. The in vitro directed evolution of DszB was initiated using error-prone PCR. An asexual dszB mutant library was generated and sixty mutant genes were …
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Cloning and characterization of MET2 in Pichia pastoris
… Pichia pastoris, has been used as a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and auxotrophic strains of P. …
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Transcriptional regulation of basal and phenobarbital-mediated expression of rat cytochrome 2B genes
… proteins (C/EBP) site by cotransfection of C/EBP expression vectors and supershift assays with C/EBP antisera.
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Immunogenic assessment of plant-produced Human papillomavirus type 16 chimaeric L1:L2 virus-like particles and the production of an encapsidated therapeutic DNA vaccine candidate
… Agrobacterium-mediated transfer. Optimisation of expression was conducted by comparing protein expression levels over several days using 4 plant expression vectors, with the highest yields obtained by targeting protein to the chloroplast or with the use of a self-replicating vector. The …
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Baculovirus surface display of influenza virus haemagglutinin and its potential as a vaccine
… influenza vaccine. Several surface display expression vectors were constructed, using either a truncated or full-length HA, to optimise glycoprotein incorporation into the budded virus (BV) envelope of three recombinant baculovirus variants: BacPAK6, BacPAK6 (high-titre) and flashBACULTRA …
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Towards the development of plant-made PsVs as potential delivery vehicles for therapeutic HPV vaccines
… to optimise Agrobacterium-mediated transient expression of BPV-1 L1 and L2 capsid proteins in Nicotiana benthamiana were explored. Approaches such as an increased acetosyringone concentration for recombinant Agrobacterium induction, a heat-shock treatment of post-infiltrated plants, and an …
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Secretion of active recombinant phytase from stably transformed soybean cells
… as assayed by β-glucuronidase reporter gene expression. A somatic embryogenesis approach was used for soybean regeneration from culture. The efficiencies of embryo induction and embryo conversion to form roots and shoots were compared in commercial soybean cultivars to identify optimal …
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A study of clinical strains of Pseudomonas Aeruginosa and the investigation of antibiotic resistance mechanisms in the multidrug resistant strain PA13
… substitution by serine at position 119. Two expression vectors were chosen to investigate the novel oxacillinase gene. One was a commercially available vector, pET-28a (Novagen) and the other was an in-house vector, pPC. The gene was successfully cloned into both vectors. Following induction …
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Design and production of a candidate universal influenza A vaccine in Nicotiana benthamiana plants
… plants via Agrobacterium-mediated transient expression. Plants are increasingly being examined as alternative recombinant protein expression systems due to their safety, scalability and rapid production times. Moreover, numerous studies suggest the use of recombinant virus-like particles …
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Investigating The Role of Prmt1 and Arginine Methylation of Hsp70 In Human Pancreatic Cancer
… the first part of this study, I found that the expression level of PRMT1 was elevated in both human and mouse pancreatic cancer tissues in immunohistochemistry analysis. The further functional studies demonstrated a pro-tumorigenic role of PRMT1 in several pancreatic cancer cell lines and mouse …
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Biophysical investigations of the interaction between substrate binding domain of Hsp40 and the RING finger domain of Retinoblastoma Binding Protein 6
… parameters. This was followed by the recombinant expression of the proteins within two difference expression vectors and E. coli bacterial strains. The SBDHsp40 protein was purified using affinity chromatography while the RING finger domain protein was purified to homogeneity by affinity and size …
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NcCyP41, a two domain neurospora crassa cyclophilin : characterization of its peptidyl-prolyl cis/trans isomerase activity ; isolation and functional analysis of two novel NcCyP41-binding proteins
… Its domains were cloned in different expression vectors and recombinant proteins purified from E. coli. Interaction of NcCyP41 and its domains with cellular proteins were then analysed by biochemical methods. <br> <br>PPIase activity of the entire protein and of its domains were tested …
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Toward a Cell-Based Assay for Cubam-Mediated Intrinsic Factor-Vitamin B12 Binding and Uptake
… pastoris) strain X-33; constructing the AMN expression plasmid and the mini Cubilin-EGFP fusion plasmid; and co-transfecting both plasmids into Ba/F3 cells to complete the model construction. Results: Yeast expression vectors and stable transformants for human, bovine, and platypus IF were …
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